On the mechanism of inhibition of cytochrome c oxidase by nitric oxide.

The Journal of Biological Chemistry
A GiuffrèM Brunori

Abstract

The mechanism of inhibition of cytochrome (cyt) c oxidase by nitric oxide (NO) has been investigated by stopped flow transient spectroscopy and singular value decomposition analysis. Following the time course of cyt c oxidation at different O2/NO ratios, we observed that the onset of inhibition: (i) is fast and at a high NO concentration is complete during the first turnover; (ii) is sensitive to the O2/NO ratio; and (iii) is independent of incubation time of the oxidized enzyme with NO. Analysis of the reaction kinetics and computer simulations support the conclusion that inhibition occurs via binding of NO to a turnover intermediate with a partially reduced cyt a3-CuB binuclear center. The inhibited enzyme has the optical spectrum typical of NO bound to reduced cyt a3. Reversal of inhibition in the presence of O2 does not involve a direct reaction of O2 with NO while bound at the binuclear center, since recovery of activity occurs at the rate of NO dissociation (k = 0.13 s-1), as determined in the absence of O2 using hemoglobin as a NO scavenger. We propose that removal of NO from the medium is associated with reactivation of the enzyme via a relatively fast thermal dissociation of NO from the reduced cyt a3-CuB center.

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T H StevensS I Chan
Nov 11, 1980·Biochemistry·G W BrudvigS I Chan
Oct 20, 1995·Archives of Biochemistry and Biophysics·Y TakeharaK Utsumi
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
Jan 1, 1994·Annual Review of Biochemistry·D S Bredt, S H Snyder
Mar 1, 1995·Biophysical Chemistry·F MalatestaM Brunori
Jan 15, 1995·European Journal of Biochemistry·T Soulimane, G Buse
Oct 28, 1994·Biochemical and Biophysical Research Communications·X J ZhaoW S Caughey
Mar 1, 1963·The Biochemical Journal·Q H GIBSON, C GREENWOOD

❮ Previous
Next ❯

Citations

Jun 21, 2005·Archives of Biochemistry and Biophysics·Yeong-Renn ChenJay L Zweier
Mar 7, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gian Giacomo CorbucciCarlo Alberto Palmerini
Sep 21, 2002·Molecular and Cellular Endocrinology·James G DrewettDavid J Hegge
Aug 25, 1999·Molecular and Cellular Endocrinology·E L KreklauJ G Drewett
Jul 13, 2000·Free Radical Biology & Medicine·C BeuneuM Lepoivre
Nov 26, 2002·Free Radical Biology & Medicine·Guy C Brown, Vilmante Borutaite
Mar 5, 2003·Free Radical Biology & Medicine·Paolo SartiMaurizio Brunori
Feb 13, 2001·Trends in Biochemical Sciences·M Brunori
Apr 11, 2001·Trends in Biochemical Sciences·M Brunori
Jan 18, 2003·Biochimica Et Biophysica Acta·Jeanie B McMillin, William Dowhan
Sep 29, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Somdatta Ghosh Dey, Abhishek Dey
Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·James P CollmanTodd A Eberspacher
Apr 10, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·G FiskumM F Beal
Dec 15, 2005·Critical Care Medicine·Hong KanMitchell S Finkel
May 10, 2007·Journal of Neurochemistry·Ludmila A VolobouevaRona G Giffard
Feb 20, 2008·Biochemistry. Biokhimii︠a︡·A N OsipovYu A Vladimirov
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·K L DavisF Murad
Sep 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ludmila A VolobouevaRona G Giffard
Jul 25, 2000·Biochemical and Biophysical Research Communications·P SartiM Brunori
Nov 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Fernando AntunesEnrique Cadenas
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·S ShivaV M Darley-Usmar
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Maria G MasonChristopher E Cooper
Jan 3, 2001·Journal of Molecular and Cellular Cardiology·P S BrookesP G Anderson
Mar 10, 2001·Free Radical Research·E CadenasA Boveris
Apr 26, 2005·FEBS Letters·Alessandro GiuffrèPaolo Sarti
Apr 3, 2012·Nitric Oxide : Biology and Chemistry·Christelle KamgaSruti Shiva
Dec 26, 2006·Neuroscience and Biobehavioral Reviews·T M Edwards, N S Rickard
Sep 13, 2011·Journal of Neuroscience Research·Ludmila A Voloboueva, Rona G Giffard
Mar 23, 2004·Analytical Biochemistry·Joaquim JaumotRaimundo Gargallo
Dec 3, 2009·Protein Science : a Publication of the Protein Society·Maurizio Brunori
Apr 17, 2007·Biochimica Et Biophysica Acta·Eric PiletMarten H Vos
Nov 22, 2005·Biochemical and Biophysical Research Communications·Matthew WhitemanJeffrey S Armstrong
Jun 2, 2015·Trends in Pharmacological Sciences·Yasuko Iwakiri, Moon Young Kim
Jun 24, 2006·Biochimica Et Biophysica Acta·Maurizio BrunoriPaolo Sarti
May 13, 1999·Biochimica Et Biophysica Acta·C E Cooper
Apr 22, 2004·Biochimica Et Biophysica Acta·Maurizio BrunoriPaolo Sarti
Dec 23, 1999·The Journal of Biological Chemistry·J J PoderosoA Boveris
Jul 20, 2004·Current Opinion in Critical Care·Mitchell P Fink

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.