On the mechanism of one-electron reduction of quinones by microsomal flavin enzymes: the kinetic analysis between cytochrome B5 and menadione

Free Radical Research Communications
T Iyanagi

Abstract

Univalent oxidation-reduction reactions coupled with the menadione (MK)/menadione semiquinone (MK.-) system were investigated by using microsomal flavin enzymes. NADPH-cytochrome P-450 reductase gave a dynamic equilibrium of oxidation-reduction of cytochrome b5 in the presence of menadione (MK), the level of which depended on the concentration of O2 and superoxide dismutase. The data suggest that the superoxide and menadione radicals are involved as an active intermediate in this system. The overall reaction at steady state appears to be composed of four main reactions, eqs. 2-5, and eqs. 2 and 4 are in equilibrium.

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Citations

Jul 26, 2002·Archives of Biochemistry and Biophysics·Henrikas NivinskasNarimantas Cenas
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Quanjiang JiChuan He
Sep 18, 2012·Archives of Biochemistry and Biophysics·Žilvinas AnusevičiusNarimantas Čėnas
Apr 16, 2009·Phytotherapy Research : PTR·Priya VenkatakrishnanR Timothy Miller
Jun 16, 2004·Archives of Biochemistry and Biophysics·Jie FuTakashi Iyanagi
Jul 31, 2002·Archives of Biochemistry and Biophysics·Zilvinas AnuseviciusNarimantas Cenas
Jan 5, 2002·Advances in Experimental Medicine and Biology·M KitamuraT Iyanagi
Nov 30, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Laura H J de HaanGerrit M Alink
Feb 22, 2000·The Journal of Biological Chemistry·J J PinkD A Boothman
Aug 10, 2016·The Journal of Biological Chemistry·Jacob BallGiovanni Gadda
Apr 21, 1995·Journal of Chromatography. B, Biomedical Applications·O Y HuF Y Wu
Aug 5, 2000·Biochimica Et Biophysica Acta·H MatsudaT Iyanagi

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