On the mechanism of quinol oxidation at the QP site in the cytochrome bc1 complex: studied using mutants lacking cytochrome bL or bH.

The Journal of Biological Chemistry
Shaoqing YangChang-An Yu

Abstract

To elucidate the mechanism of bifurcated oxidation of quinol in the cytochrome bc1 complex, Rhodobacter sphaeroides mutants, H198N and H111N, lacking heme bL and heme bH, respectively, were constructed and characterized. Purified mutant complexes have the same subunit composition as that of the wild-type complex, but have only 9-11% of the electron transfer activity, which is sensitive to stigmatellin or myxothiazol. The Em values for hemes bL and bH in the H111N and H198N complexes are -95 and -35 mV, respectively. The pseudo first-order reduction rate constants for hemes bL and bH in H111N and H198N, by ubiquiniol, are 16.3 and 12.4 s(-1), respectively. These indicate that the Qp site in the H111N mutant complex is similar to that in the wild-type complex. Pre-steady state reduction rates of heme c1 by these two mutant complexes decrease to a similar extent of their activity, suggesting that the decrease in electron transfer activity is due to impairment of movement of the head domain of reduced iron-sulfur protein, caused by disruption of electron transfer from heme bL to heme bH. Both mutant complexes produce as much superoxide as does antimycin A-treated wild-type complex. Ascorbate eliminates all superoxide generating act...Continue Reading

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Citations

May 11, 2011·Nature Communications·Alizée MalnoëFabrice Rappaport
Apr 8, 2010·The Journal of Biological Chemistry·Ying YinChang-An Yu
Dec 19, 2012·Antioxidants & Redox Signaling·Tiago R FigueiraAnibal E Vercesi
Aug 28, 2012·Biochimica Et Biophysica Acta·Fei ZhouChang-An Yu
Feb 12, 2013·Biochimica Et Biophysica Acta·Antony R CroftsKlaus Schulten

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