On the mechanism of RecA-mediated repair of double-strand breaks: no role for four-strand DNA pairing intermediates

Molecular Cell
Q Shan, M M Cox

Abstract

RecA protein will bind to a gapped duplex DNA molecule and promote a DNA strand exchange with a second homologous linear duplex. A double-strand break in the second duplex is efficiently bypassed in the course of these reactions. We demonstrate that the bypass of double-strand breaks is not explained by a mechanism involving homologous interactions between two duplex DNA molecules, but instead requires the ATP-mediated generation of DNA torsional stress brought about by the action of RecA. The results suggest new pathways for the repair of double-strand breaks and underline the need for new paradigms to explain the alignment of homologous DNAs during genetic recombination.

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Citations

Jan 18, 2007·Nature Reviews. Molecular Cell Biology·Michael M Cox
Jul 8, 2008·The Journal of Biological Chemistry·Julia M CoxMichael M Cox
Jun 5, 2002·Annual Review of Biochemistry·Shelley L Lusetti, Michael M Cox
Feb 19, 2005·PLoS Biology·Julia M CoxMichael M Cox
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M E RobuM M Cox
Apr 1, 2015·Nucleic Acids Research·Vessela PetrovaMichael M Cox
Oct 7, 2003·Annual Review of Microbiology·Michael M Cox
Feb 25, 2003·The Journal of Biological Chemistry·Shelley L LusettiMichael M Cox
Oct 8, 2003·The Journal of Biological Chemistry·Nami HarutaMichael M Cox

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