PMID: 7009752Jan 1, 1980Paper

On the mutagenic action of some enzyme immunoassay substrates

Journal of Immunological Methods
C E VoogdJ J Jacobs

Abstract

The mutagenic action of six compounds used in ELISA, EMIT and EIA assays, was investigated by means of the fluctuation test, with Klebsiella pneumoniae as a test organism, and the Ames' plate incorporation test using Salmonella typhimurium TA 98, TA 100 and TA 1537. It appears that 2,2'-azino-di(3-ethyl-benzthiazoline sulphonic acid (6)) or ABTS exerts mutagenic action on Klebsiella pneumoniae at a concentration of 11 g/l, on Salmonella typhimurium TA 100 at a top agar concentration of 0.1 g/l, on Salmonella typhimurium TA 98 at 0.2 g/l and on Salmonella typhimurium TA 1537 at 10 g/l. With umbelliferone, mutagenic action was found only with Klebsiella pneumoniae at a concentration of 0.8 g/l or higher. With o-phenylenediamine, strong mutagenic activity was found only with strain TA 98 and metabolic activation at a top agar concentration of 0.001 g/l. With 5-aminosalicylic acid, beta-methylumbelliferone and p-nitrophenyl-phosphate, no mutagenic action was observed.

Citations

Oct 26, 2012·Environmental Science and Pollution Research International·Jahir Orozco, Linda K Medlin
Oct 12, 1984·Journal of Immunological Methods·R H YolkenM Van Regenmortel
Jul 1, 1986·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·M Gunasekaran, T Sambandam
Jan 27, 2009·Biosensors & Bioelectronics·Charlie O Parker, Ibtisam E Tothill
May 21, 2009·Journal of Clinical Laboratory Analysis·Lílian Dias NascimentoMauro Célio de Almeida Marzochi
Nov 1, 1992·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C R Taylor
Apr 7, 2021·Journal of Microbiology and Biotechnology·Jiyon LeeDo-Young Yoon
Aug 5, 1989·Biotechnology and Bioengineering·M GarnP Cevey

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