On the possibility of real-time monitoring of glucose in cell culture by microdialysis using a fluorescent glucose binding protein sensor

Biotechnology Progress
Xudong GeLeah Tolosa

Abstract

Although glucose sensors with millimolar sensitivity are still the norm, there is now a developing interest in glucose sensors with micromolar sensitivity for applications in minimally invasive sampling techniques such as fast microdialysis and extraction of interstitial fluid by iontophoresis and laser poration. In this regard, the glucose binding protein (GBP) with a binding constant for glucose in the micromolar range is of particular relevance. GBP is one of the soluble binding proteins found in the periplasmic space of Gram-negative bacteria. Because of its hinge-like tertiary structure, glucose binding induces a large conformational change, which can be used for glucose sensing by attaching a polarity sensitive fluorescent probe to a site on the protein that is allosterically responsive to glucose binding. Correspondingly, the resulting optical biosensor has micromolar sensitivity to glucose. Because binding is reversible, the biosensor is reusable and can be stored at 4 degrees C for 6 months without losing its sensitivity. In this paper, we show the feasibility of using the GBP biosensor to monitor glucose in microdialysis. The effect of perfusion rate, bulk glucose concentration and temperature on microdialysis efficie...Continue Reading

Citations

Sep 26, 2013·Archives of Disease in Childhood. Fetal and Neonatal Edition·Hyung Chul WooRose M Viscardi
Mar 6, 2010·Biotechnology Progress·Bhargavi KondraguntaGovind Rao
Sep 20, 2017·International Journal of Molecular Sciences·Alexander V FoninKonstantin K Turoverov
May 3, 2019·PloS One·Tarini Nawamalie Abeysinghe GunawardenaNoor Hayaty Abu Kasim
Mar 4, 2014·Measurement Science & Technology·Yordan KostovLeah Tolosa
Sep 2, 2018·Analytical and Bioanalytical Chemistry·Sheniqua BrownLeah Tolosa
Feb 22, 2011·Biosensors & Bioelectronics·Sha JinKaiming Ye

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