On the potential for fibronectin/phosphorylcholine coatings on PTFE substrates to jointly modulate endothelial cell adhesion and hemocompatibility properties

Biomatter
Vanessa Montaño-MachadoEmmanuel Pauthe

Abstract

The use of biomolecules as coatings on biomaterials is recognized to constitute a promising approach to modulate the biological response of the host. In this work, we propose a coating composed by 2 biomolecules susceptible to provide complementary properties for cardiovascular applications: fibronectin (FN) to enhance endothelialization, and phosphorylcholine (PRC) for its non thrombogenic properties. Polytetrafluoroethylene (PTFE) was selected as model substrate mainly because it is largely used in cardiovascular applications. Two approaches were investigated: 1) a sequential adsorption of the 2 biomolecules and 2) an adsorption of the protein followed by the grafting of phosphorylcholine via chemical activation. All coatings were characterized by immunofluorescence staining, X-Ray Photoelectron Spectroscopy and Scanning Electron Microscopy analyses. Assays with endothelial cells showed improvement on cell adhesion, spreading and metabolic activity on FN-PRC coatings compared with the uncoated PTFE. Platelets adhesion and activation were both reduced on the coated surfaces when compared with uncoated PTFE. Moreover, clotting time tests exhibited better hemocompatibility properties of the surfaces after a sequential adsorption...Continue Reading

References

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Citations

Jul 21, 2017·Tissue Engineering. Part a·Ji-Yun KoGun-Il Im
Apr 27, 2019·Frontiers in Bioengineering and Biotechnology·Francesco CopesDiego Mantovani
May 16, 2020·Journal of Colloid and Interface Science·Weiwei ZhengDaidi Fan
Dec 8, 2019·International Journal of Biological Macromolecules·Renata Francielle Bombaldi de SouzaÂngela Maria Moraes

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