On the potential of electrochemically modulated liquid chromatography of proteins in a micro open parallel plate separator

Journal of Separation Science
Blanca H Lapizco-Encinas, Neville G Pinto

Abstract

Electrochemically Modulated Liquid Chromatography (EMLC) is a technique in which an electrical potential is applied to the stationary phase in order to alter its adsorptive properties. The micro Open Parallel Plate Separator (microOPPS) is an open-channel micro-chromatographic system with the unique characteristic of an adjustable rectangular flow cross-section. In this publication, the performance of the microOPPS in the EMLC mode was simulated using a comprehensive mathematical model. The separation of two proteins was analyzed by performing a parametric study for the effects of the potential applied to the stationary phase, the dimensions of the device, and the operating conditions. It is shown that the combination of the microOPPS and EMLC is promising for protein separations. These separations can be optimized by carefully selecting the applied potential and the geometry of the microOPPS. The marriage of the microOPPS and EMLC adds two additional dimensions for control, to improve the separation efficiency in analytical applications and in preparative separations of trace compounds.

References

Oct 1, 1994·Biophysical Chemistry·J A GerstnerS M Cramer
Feb 5, 2000·Analytical Chemistry·P LamT M Przybycien
Feb 20, 1990·Biotechnology and Bioengineering·M T Tyn, T W Gusek

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Citations

Dec 30, 2016·Journal of Separation Science·Markus BrammenSonja Berensmeier
Oct 23, 2019·Analytical Chemistry·Robert J SotoMarc D Porter

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