On the question of integration of Agrobacterium tumefaciens deoxyribonucleic acid by tomato plants.

Journal of Bacteriology
R S Hanson, M D Chilton

Abstract

Treatment of tomato plants with Agrobacterium tumefaciens causes subsequently administered [3H]thymidine to be preferentially incorporated into a satellite deoxyribonucleic acid (DNA) whose buoyant density is between that of bacterial DNA (rho = 1.718 g/cm3) and plant main band DNA (rho = 1.692 g/cm3). Satellite DNA upon shearing or sonic treatment releases fragments of higher and lower buoyant density, as reported by earlier investigators. The satellite has no significant base sequence homology with A. tumefaciens DNA, for its rate of reassociation is not accelerated by the addition of high concentrations of the latter. Tomato DNA isolated from shoots or from leaf nuclei accelerates renaturation of labeled satellite DNA. We conclude that the intermediate density labeled DNA is a plant satellite and not the product of covalent joining of bacterial and plant DNA as suggested by earlier investigators.

References

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·A KleinhofsA J Bendich
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R Kolodner, K K Tewari
Dec 19, 1973·Nature: New Biology·R ParentiM Buiatti
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M D ChiltonE W Nester
Apr 18, 1973·Nature: New Biology·J IngleJ Sinclair
Apr 10, 1974·Biochimica Et Biophysica Acta·F Vedel, F Quetier
Jan 1, 1971·Molecular & General Genetics : MGG·M Stroun, P Anker
Mar 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·A H WhiteleyH R Whiteley

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