PMID: 2496984May 1, 1989Paper

On the role of phospholipids in the reconstituted cytochrome P-450 system. A model study using dilauroyl and distearoyl glycerophosphocholine

European Journal of Biochemistry
I M RietjensC Veeger

Abstract

The difference in pentoxyresorufin O-dealkylating activity observed in a reconstituted system containing dilauroylglycerophosphocholine (Lau2GroPCho) or distearoylglycerophosphocholine (Ste2GroPCho) was used as a model to study the role of phospholipids in the reconstituted cytochrome P-450b (IIB1) system. The hypotheses proposed in the literature for the role of phospholipids in the reconstituted cytochrome P-450 system, mainly based on the comparison of systems without phospholipid and with Lau2GroPCho, were either validated or shown to be unlikely when tested by comparing reconstituted systems with different phosphatidylcholines. The higher activity in the Lau2GroPCho system as compared to the Ste2GroPCho system cannot be ascribed to (a) an increased affinity of cytochrome P-450 for the NADPH-cytochrome reductase in the Lau2GroPCho system, also not to (b) a Lau2GroPCho-dependent dissociation of protein multimers, nor to (c) a change in the spin state of the heme. We found a different apparent Km for pentoxyresorufin in the Lau2GroPCho system compared with the Ste2GroPCho system. Furthermore, we found a difference between the cytochrome P-450b tryptophan fluorescence polarization of the Lau2GroPCho system and the Ste2GroPCho ...Continue Reading

References

Feb 14, 1984·Biochemical and Biophysical Research Communications·H TaniguchiR Sato

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Citations

Jan 1, 1991·The International Journal of Biochemistry·T Cserháti, M Szögyi
Mar 1, 1996·Journal of Hepatology·A PastorJ González-Gallego
Oct 9, 1998·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J G BessemsN P Vermeulen
Dec 15, 1993·European Journal of Biochemistry·W G BalversI M Rietjens
May 13, 2009·Chembiochem : a European Journal of Chemical Biology·Antoinette ChougnetBoris Schilling
Aug 15, 1995·European Journal of Biochemistry·C DelormeA Marquet

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