PMID: 7011814Feb 1, 1981Paper

On the role of ribosylthymine in prokaryotic tRNA function

European Journal of Biochemistry
H KerstenK H Nierhaus

Abstract

tRNAPhe and tRNALys were isolated from an Escherichia coli K12 mutant deficient in ribosylthymine (rT) and from the wild-type strain. The sequence G-rT-psi-C which is common to loop IV of practically all tRNAs used in the elongation cycle of protein synthesis reads G-U-psi-C in the tRNAs of the mutant strain. The purified tRNAs were compared in various steps of protein biosynthesis. The poly(U)-dependent poly(Phe) synthesis performed with purified Phe-tRNAPhe and purified elongation factors showed no dependence on the presence or absence of ribosylthymine in the respective tRNAs. In contrast, the corresponding poly(A)-dependent poly(Lys) synthesis was markedly increased when Lys-tRNALys lacking rT was used. The analysis of individual functional steps of the poly(A)-dependent elongation cycle demonstrated that the absence of rT reduced the binding to the A-site and improved the translocation reaction, whereas the formation of the ternary complex EF-Tu . GTP . aa-tRNA as well as both tRNA binding to the P-site and the peptidyltransferase reaction remained unaffected. The presence of U in place of rT in tRNA increases the misincorporation of leucine in an optimized poly(U)/poly(Phe) system from about 3 in 10 000 to 3 in 1000. Our ...Continue Reading

References

Jan 1, 1977·Cell·P Edelmann, J Gallant
May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Wurmbach, K H Nierhaus
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B A Roe, H Y Tsen
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P C Jelenc, C G Kurland
Jan 1, 1979·Methods in Enzymology·K H Nierhaus, F Dohme
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A MöllerH G Gassen
Jul 13, 1976·Biochemistry·M SprinzlV A Erdmann
Jun 18, 1976·Biochimica Et Biophysica Acta·R RaettigH H Arnold
Jul 1, 1973·Archives of Biochemistry and Biophysics·S YangM L Gefter
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G Petrissant
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·M SimsekU L Rajbhandary
May 10, 1972·Nature: New Biology·R J Roberts
Jun 28, 1972·Journal of Molecular Biology·S Watanabe
Aug 1, 1972·The Biochemical Journal·R B Loftfield, D Vanderjagt
Jan 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·J Gordon
May 13, 1971·Biochimica Et Biophysica Acta·I SvenssonA Henningsson
Jan 18, 1966·Biochimica Et Biophysica Acta·A L Haenni, F Chapeville
Mar 1, 1966·Journal of Molecular Biology·R F Gesteland
Jan 11, 1980·Nucleic Acids Research·B BowenH Weintraub
Sep 25, 1980·Nucleic Acids Research·M E Dobson, V M Ingram
Jan 1, 1974·Nucleic Acids Research·H J GrossU L Raj Bhandary
Oct 1, 1963·The Biochemical Journal·R B LOFTFIELD

❮ Previous
Next ❯

Citations

May 11, 2002·RNA·Marcus J O Johansson, Anders S Byström
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B C PerssonG R Björk
May 1, 1990·DNA and Cell Biology·X R Gu, D V Santi
Nov 2, 2005·Protein Science : a Publication of the Protein Society·Warren SunDinesh Christendat
May 1, 2014·EcoSal Plus·Glenn R Björk, Tord G Hagervall
Nov 1, 2005·EcoSal Plus·Glenn R Björk, Tord G Hagervall
Aug 3, 1993·Biochemistry·K M HarringtonO C Uhlenbeck

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.