On the structure of polymeric IgM

European Journal of Immunology
A C DavisM J Shulman

Abstract

The cysteine at position 575 of the immunoglobulin mu heavy chain is thought to provide the only disulfide bonds joining the monomer subunits of mouse polymeric IgM. The importance of this cysteine in the assembly of polymeric IgM was investigated by using site-directed mutagenesis to produce mu chains with serine at position 575. Thirty percent of the secreted mutant IgM was covalently assembled polymer implying that cysteines other than Cys575 can form inter-subunit disulfide bonds. The polymeric IgM lacked J chain, mediated complement-dependent cytolysis and appeared to have a higher molecular weight than conventional IgM pentamers, as judged by sucrose gradient sedimentation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis mobility. Electron microscopy revealed that the mutant IgM molecule contained six subunits. Wild-type IgM, while synthesized predominantly as a pentameric molecule, was assembled in at least two other forms, which were distinguished by their electrophoretic mobility. The apparently higher molecular weight forms of wild-type IgM include hexameric molecules which, like the hexameric mutant IgM, contained much less J chain that the pentameric form and were 20-fold more efficient at activating co...Continue Reading

References

Feb 1, 1978·European Journal of Immunology·G KöhlerM J Shulman
Dec 1, 1975·The Biochemical Journal·C P MilsteinA Feinstein
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M J ShulmanN Hozumi
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K H RouxN S Greenspan
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R M Chapuis, M E Koshland
Jun 14, 1974·Nature·J Mestecky, R E Schrohenloher
Apr 1, 1969·The Biochemical Journal·D Beale, A Feinstein
Dec 26, 1970·Nature·M S Halpern, M E Koshland
Jan 1, 1983·Methods in Enzymology·M J Zoller, M Smith
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A OchiN Hozumi
Sep 1, 1982·Molecular and Cellular Biology·M J ShulmanG Köhler
Jun 1, 1986·Immunology Today·A FeinsteinM I Taussig

Citations

Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P S Reddy, R B Corley
Aug 26, 1998·European Journal of Immunology·L ErlandssonT Leanderson
Jul 1, 1993·Somatic Cell and Molecular Genetics·M D Baker, L R Read
Jul 26, 2011·Immunologic Research·Vladimir PetrušićLjiljana Dimitijević
Feb 1, 1989·Molecular Immunology·A C DavisM J Shulman
Apr 1, 1994·Immunology Today·J W BrewerR B Corley
Feb 1, 1995·Current Opinion in Immunology·V D Miletić, M M Frank
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T D RandallR B Corley
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·K D BornemannH M Jäck
Mar 27, 2007·Future Oncology·M H RavindranathD L Morton
Mar 29, 2000·Proceedings of the National Academy of Sciences of the United States of America·C L CheungC M Lieber
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Roger MüllerJohannes Buchner
Dec 3, 2015·Biotechnology Advances·Nikolay VasilevAndreas Schiermeyer
Jul 16, 2011·Molecular Immunology·Nina A DahaLeendert A Trouw
Dec 26, 2006·Biochemical and Biophysical Research Communications·M H RavindranathD L Morton
Jul 15, 2005·Annals of the New York Academy of Sciences·M H RavindranathD L Morton
Dec 2, 2004·Journal of Autoimmunity·Michele E YoudR B Corley
Jul 21, 2004·Molecular Immunology·Kathryn E GunnJ W Brewer
Apr 3, 2016·Journal of the American Society for Mass Spectrometry·Edward S X MohNicolle H Packer
Sep 12, 2012·Immunobiology·Christian M Karsten, Jörg Köhl
Sep 10, 2011·Medical Hypotheses·Vladimir PetrušićLjiljana Dimitrijević
Nov 20, 2016·Scandinavian Journal of Immunology·T E MichaelsenI Sandlie
Dec 23, 1999·International Immunology·V SørensenI Sandlie
Jan 19, 2018·Journal of Leukocyte Biology·Gretchen Harms Pritchard, Marion Pepper
Jun 16, 2005·The Journal of Biological Chemistry·James N ArnoldR B Sim
Sep 23, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Caitlin D Castro, Martin F Flajnik
Sep 1, 1990·European Journal of Immunology·T D RandallR B Corley
Aug 28, 2020·Frontiers in Immunology·Siqi Gong, Ruth M Ruprecht
Jan 8, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandra BonnerStephen J Perkins
Oct 18, 2020·Antibodies·Bruce A KeytMarvin S Peterson
Nov 17, 2020·Frontiers in Immunology·Katelyn JonesSabelo Hadebe
Mar 7, 2021·International Journal of Molecular Sciences·Hao Wei, Ji-Yang Wang

Related Concepts

Immunoglobulin M pentamer
Cysteine Hydrochloride
Disulfides
DNA Mutational Analysis
SDS-PAGE
Hybridomas
IgM2
Immunoglobulin J-Chains
Electron Microscopy
Protein Conformation

Related Feeds

Antibody Engineering

Antibody engineering technologies are constantly advancing to improve the clinical effectiveness of monoclonal and bispecific antibodies. Discover the latest research on Antibody Engineering here.