Mar 22, 2020

CryoGAN: A New Reconstruction Paradigm for Single-particle Cryo-EM Via Deep Adversarial Learning

BioRxiv : the Preprint Server for Biology
Harshit GuptaM. Unser


We present CryoGAN, a new paradigm for single-particle cryo-EM reconstruction based on unsupervised deep adversarial learning. The major challenge in single-particle cryo-EM is that the measured particles have unknown poses. Current reconstruction techniques either estimate the poses or marginalize them away--steps that are computationally challenging. CryoGAN sidesteps this problem by using a generative adversarial network (GAN) to learn the 3D structure whose simulated projections most closely match the real data in a distributional sense. The architecture of CryoGAN resembles that of standard GAN, with the twist that the generator network is replaced by a cryo-EM physics simulator. CryoGAN is an unsupervised algorithm that only demands picked particle images and CTF estimation as inputs; no initial volume estimate or prior training are needed. Moreover, it requires minimal user interaction and can provide reconstructions in a matter of hours on a high-end GPU. Experiments on synthetic datasets confirm that CryoGAN can reconstruct a high-resolution volume with its adversarial learning scheme. Preliminary results on real {beta}-galactosidase data demonstrate its ability to capture and exploit real data statistics in more chall...Continue Reading

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Mentioned in this Paper

Gene Polymorphism
Haploid Cell
Diploid Cell
Genetic Polymorphism
Orientation (spatial)

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