On the suitability of refractometry for the analysis of glucose in blood-derived fluids

Medical Engineering & Physics
Kai Zirk, Harald Poetzschke

Abstract

Refractometry is the determination of the optical refractive index of a substance or a mixture of substances. It is a very sensitive method for the detection and quantification of dissolved analytes, but it is incapable of distinguishing between different analytes. The aim of this investigation was to determine the principle suitability of refractometry for the quantification of glucose (blood sugar) in blood and various blood fluids which can readily be obtained for medical diagnosis, in particular blood plasma, blood serum, and their ultrafiltrates. After the oral intake of freshly dissolved alpha-glucose, the in vivo blood contents of the alpha and beta anomers of glucose were found to be in an at least approximate equilibrium at all times. This observation is a prerequisite for a refractometrical determination of glucose due to the fact that both molecule forms have different refractive index increments. An assessment of the glucose content in untreated blood fluids was not possible, since no suitable relationship to the refractive index was found, most probably due to the influence of the many other substances present in blood on this parameter. However, after removal of certain macromolecules by ultrafiltration, value pai...Continue Reading

Citations

Jul 2, 2009·Journal of Biomedical Optics·Joerg MartiniRichard H Bruce
Aug 1, 2006·Medical Engineering & Physics·Kai Zirk, Harald Poetzschke
Nov 10, 2016·Applied Optics·L F G Dib, E A Barbosa
Feb 29, 2020·Sensors·Maryamsadat Shokrekhodaei, Stella Quinones
Jul 3, 2020·The Review of Scientific Instruments·Manuel TrierweilerCornelius Neumann
Dec 10, 2020·Sensors·Liu TangJen-Tsai Liu

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