On the temperature and tension dependence of the outer hair cell lateral membrane conductance GmetL and its relation to prestin

Pflügers Archiv : European journal of physiology
J Santos-SacchiDhasakumar Navaratnam

Abstract

Recently, we identified an outer hair cell (OHC) lateral membrane conductance, GmetL, that colocalizes with prestin and passes Cl-, thereby influencing prestin's (SLC26A5) electromechanical activity. In this study, we report on a comparison of the temperature and tension dependence of GmetL and prestin. Though we find significant temperature and tension dependence of each, substantial differences exist which indicate their independent identity. The following data support this conclusion: (1) The voltage dependence of GmetL does not follow that of prestin's nonlinear capacitance (NLC) function when the latter is shifted by either temperature or membrane tension; (2) Unlike native OHCs whose NLC can be modulated by influx of extracellular Cl-, prestin-transfected Chinese hamster ovary (CHO) cells do not show this phenomenon; (3) Stretch-sensitive, single channel currents are not evidenced after prestin transfection in CHO cells; and (4) There is no correlation between prestin expression level (gauged via NLC) and transmembrane current through GmetL. Thus, GmetL must result from the activity of another molecular species within the lateral membrane of the OHC. A clue to its identity is the conductance's nonlinear temperature depend...Continue Reading

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Aug 23, 2005·Biophysical Journal·Dhasakumar NavaratnamJoseph Santos-Sacchi

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Citations

Apr 20, 2017·Scientific Reports·Jun-Ping BaiDhasakumar Navaratnam
Jan 16, 2008·Physiological Reviews·Jonathan Ashmore

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