On the use of ion-pair chromatography to elucidate doxorubicin release mechanism from polyalkylcyanoacrylate nanoparticles at the cellular level

Journal of Chromatography. B, Biomedical Sciences and Applications
X PepinP Couvreur

Abstract

The major hypothesis underlying the remarkable efficiency of polyalkylcyanoacrylate particles loaded with doxorubicin against multidrug resistant tumor cells in vitro, is based on the ion-pair association of doxorubicin with soluble hydrolysis products of polyalkylcyanoacrylate. In an attempt to demonstrate the validity of this hypothesis, we have used ion-pair reversed-phase high-performance liquid chromatography and a laboratory-synthetized compound, i.e., the 2-cyano-2-butylhexanoic acid, as a model for polyalkylcyanoacrylate highly polydispersed degradation products. It is shown that, compared to a counter-ion, like heptane sulfonic acid, 2-cyano-2-butylhexanoic acid exhibits an effective ion-pairing effect at different pH values and organic mobile phase conditions. Moreover, at pH close to physiological conditions and at low mobile phase organic modifier percentage, this effect is experimentally observed, which strongly supports the initial hypothesis.

References

Aug 4, 1992·Biochemical Pharmacology·C CuvierM F Poupon
Mar 1, 1984·Biomaterials·V LenaertsP Speiser
Jan 1, 1994·Cancer Chemotherapy and Pharmacology·A Colin de VerdièreP Couvreur

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Citations

Jun 16, 2010·Pharmaceutical Research·Jeremy D Heidel, Mark E Davis
Nov 26, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·C VauthierP Couvreur
Sep 3, 2002·Advanced Drug Delivery Reviews·Irène BriggerPatrick Couvreur
Apr 23, 2003·Advanced Drug Delivery Reviews·Christine VauthierPatrick Couvreur
Jun 10, 2010·Nanomedicine·Xiaowei Dong, Russell J Mumper
Aug 19, 2009·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Xu WangDong M Shin
Oct 31, 2006·Journal of Biomedical Materials Research. Part a·Xuemei WangBaoan Chen
May 14, 2014·Advanced Drug Delivery Reviews·Mathumai KanapathipillaiDonald E Ingber
Feb 6, 2019·Journal of Drug Targeting·Simona MuraJulien Nicolas
Nov 2, 2005·The Journal of Pharmacy and Pharmacology·L Harivardhan Reddy
Sep 2, 2008·Nature Reviews. Drug Discovery·Mark E DavisDong M Shin
Jul 1, 2008·Critical Reviews in Analytical Chemistry·Teresa Cecchi

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