On-tissue protein identification and imaging by MALDI-ion mobility mass spectrometry.

Journal of the American Society for Mass Spectrometry
Jonathan StauberRon M A Heeren

Abstract

MALDI imaging mass spectrometry (MALDI-IMS) has become a powerful tool for the detection and localization of drugs, proteins, and lipids on-tissue. Nevertheless, this approach can only perform identification of low mass molecules as lipids, pharmaceuticals, and peptides. In this article, a combination of approaches for the detection and imaging of proteins and their identification directly on-tissue is described after tryptic digestion. Enzymatic digestion protocols for different kinds of tissues--formalin fixed paraffin embedded (FFPE) and frozen tissues--are combined with MALDI-ion mobility mass spectrometry (IM-MS). This combination enables localization and identification of proteins via their related digested peptides. In a number of cases, ion mobility separates isobaric ions that cannot be identified by conventional MALDI time-of-flight (TOF) mass spectrometry. The amount of detected peaks per measurement increases (versus conventional MALDI-TOF), which enables mass and time selected ion images and the identification of separated ions. These experiments demonstrate the feasibility of direct proteins identification by ion-mobility-TOF IMS from tissue. The tissue digestion combined with MALDI-IM-TOF-IMS approach allows a pr...Continue Reading

References

Jan 15, 1999·Journal of the American Society for Mass Spectrometry·M StoeckliR M Caprioli
Jul 17, 1999·Journal of Mass Spectrometry : JMS·L HanleyJ L Trevor
Feb 3, 2000·Analytical Chemistry·R W Garden, J V Sweedler
Mar 21, 2003·Journal of Proteome Research·Brandon T RuotoloDavid H Russell
Apr 15, 2004·Analytical Chemistry·Amina S WoodsJ Albert Schultz
Sep 15, 2004·Analytical Chemistry·Stefan L LuxembourgRon M A Heeren
Dec 22, 2004·Mechanisms of Ageing and Development·Tatiana C RohnerMarkus Stoeckli
Apr 14, 2005·Journal of the American Chemical Society·John A McLeanDavid H Russell
Aug 13, 2005·Journal of the American Society for Mass Spectrometry·John C JurchenJonathan V Sweedler
Aug 16, 2006·Analytical Chemistry·Praneeth D EdirisingheLuke Hanley
Oct 24, 2006·Journal of the American Society for Mass Spectrometry·Ioana M TabanGökhan Baykut
Jan 19, 2007·Journal of Mass Spectrometry : JMS·M Reid GrosecloseRichard M Caprioli
Feb 13, 2007·Journal of Proteome Research·R LemaireI Fournier
May 2, 2007·Mass Spectrometry Reviews·Liam A McDonnell, Ron M A Heeren
Jul 11, 2007·Journal of Mass Spectrometry : JMS·Shelley N JacksonAmina S Woods
Nov 10, 2007·Molecular & Cellular Proteomics : MCP·Pierre ChaurandSusan Kasper
Jan 2, 2008·Analytical Chemistry·Shuichi ShimmaMitsutoshi Setou
Feb 8, 2008·Rapid Communications in Mass Spectrometry : RCM·Panos Hatsis, James T Kapron
Jan 13, 2010·European Journal of Mass Spectrometry·Martin FroeschRon M A Heeren

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Citations

Dec 29, 2010·Analytical and Bioanalytical Chemistry·Maria Careri, Alessandro Mangia
Jan 13, 2011·Analytical and Bioanalytical Chemistry·András Kiss, Ron M A Heeren
Aug 17, 2010·Der Pathologe·K-F BeckerH Höfler
Oct 29, 2010·Histochemistry and Cell Biology·Jaroslav PólMichael Volný
May 9, 2013·Histochemistry and Cell Biology·Andreas Römpp, Bernhard Spengler
Sep 11, 2010·Sexual Plant Reproduction·Ján A MiernykMartin Hajduch
Sep 21, 2013·Journal of the American Society for Mass Spectrometry·Wei RaoDavid A Barrett
Apr 29, 2010·Chemical Reviews·Kamila Chughtai, Ron M A Heeren
Sep 29, 2011·Chemical Reviews·Karin A Zemski BerryRobert C Murphy
Jul 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Na Young HaSang Yun Han
Jun 15, 2011·Journal of Proteome Research·Maurizio RonciNicolas H Voelcker
Aug 24, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mohammadreza ShariatgorjiPer E Andrén
Aug 2, 2011·Molecular BioSystems·Weichao GuoBaohong Liu
Dec 15, 2010·Molecular & Cellular Proteomics : MCP·Mélanie LagarrigueCharles Pineau
Aug 26, 2010·Molecular & Cellular Proteomics : MCP·Pierre ChaurandRichard M Caprioli
May 27, 2011·Thrombosis and Haemostasis·Aurélien ThomasChristian Staub
Dec 24, 2011·Bioanalysis·Stacey R Oppenheimer, Dieter M Drexler
Feb 8, 2013·The Review of Scientific Instruments·Andras KissRon M A Heeren
Oct 20, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hui YeLingjun Li
Apr 25, 2014·Journal of the American Society for Mass Spectrometry·Roberto FernándezJosé A Fernández
Jul 23, 2013·Bioinformatics·Theodore Alexandrov, Andreas Bartels
May 9, 2014·Bioanalysis·Aurélien Thomas, Pierre Chaurand
Apr 2, 2014·Journal of Proteomics·Megan M GesselRichard M Caprioli
Sep 15, 2015·Archives of Biochemistry and Biophysics·Curt R FischerBenjamin P Bowen
Oct 16, 2012·Clinical Biochemistry·David Cilia Vincenti, Graeme I Murray
Apr 4, 2012·Journal of Proteomics·Julia H Jungmann, Ron M A Heeren
Mar 29, 2011·Biochimica Et Biophysica Acta·Naoko Goto-InoueMitsutoshi Setou
Dec 22, 2010·Journal of Proteomics·Ján A Miernyk, Martin Hajduch
Sep 17, 2015·Journal of the American Society for Mass Spectrometry·Fabrizio DonnarummaKermit K Murray
Nov 7, 2015·Journal of Proteome Research·Bram HeijsLiam A McDonnell
Jan 4, 2012·Proteomics. Clinical Applications·Alessandro TancaMaria Filippa Addis
Apr 3, 2012·Rapid Communications in Mass Spectrometry : RCM·Yvonne SchoberAndreas Römpp
Nov 19, 2011·Rapid Communications in Mass Spectrometry : RCM·Filip CuyckensJan Claereboudt
Jan 1, 2014·Proteomics·Rima Ait-BelkacemDaniel Lafitte
Oct 11, 2014·Journal of Mass Spectrometry : JMS·Mathieu GaudinOlivier Laprévote

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