Oncogenic Met receptor induces ectopic structures in Xenopus embryos

Oncogene
Akihiko IshimuraIra O Daar

Abstract

When aberrantly expressed or activated, the Met receptor tyrosine kinase is involved in tumor invasiveness and metastasis. In this study, we have used the Xenopus embryonic system to define the role of various Met proximal-binding partners and downstream signaling pathways in regulating an induced morphogenetic event. We show that expression of an oncogenic derivative of the Met receptor (Tpr-Met) induces ectopic morphogenetic structures during Xenopus embryogenesis. Using variant forms of Tpr-Met that are engineered to recruit a specific signaling molecule of choice, we demonstrate that the sole recruitment of either the Grb2 or the Shc adaptor protein is sufficient to induce ectopic structures and anterior reduction, while the recruitment of PI-3Kinase (PI-3K) is necessary but not sufficient for this effect. In contrast, the recruitment of PLCgamma can initiate the induction, but fails to maintain or elongate supernumerary structures. Finally, evidence indicates that the Ras/Raf/MAPK pathway is necessary, but not sufficient to induce these structures. This study also emphasizes the importance of examining signaling molecules in the regulatory context that is provided by receptor/effector interactions when assessing a role in ...Continue Reading

References

May 21, 1992·Nature·M Whitman, D A Melton
Jan 1, 1990·Vision Research·A M NorciaR D Hamer
Jun 1, 1985·Biochemical Pharmacology·T TakenawaY Nagai
Aug 1, 1986·Developmental Biology·M Jacobson, U Rutishauser
Feb 1, 1987·Molecular and Cellular Biology·M DeanG F Vande Woude
Nov 1, 1981·The British Journal of Ophthalmology·R L Radius, D R Anderson
Aug 1, 1995·Current Opinion in Genetics & Development·T P Yamaguchi, J Rossant
Jan 1, 1994·Cold Spring Harbor Symposia on Quantitative Biology·S Rong, G F Vande Woude
Apr 22, 1994·Cell·A Hemmati-Brivanlou, D A Melton
Oct 1, 1995·Cell Biology and Toxicology·M GovernaG Scansetti
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·H TakayamaG Merlino
May 31, 1996·The Journal of Biological Chemistry·E D FixmanM Park
Jun 14, 1996·The Journal of Biological Chemistry·C PonzettoP Comoglio
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·H TakayamaG Merlino
May 8, 1997·Biochemical and Biophysical Research Communications·S AokiT Nakamura
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·M JeffersG F Vande Woude
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·K NakataK Mikoshiba
Jan 1, 1997·Annual Review of Cell and Developmental Biology·R Harland, J Gerhart
Jun 23, 1998·Current Biology : CB·M E PownallJ M Slack
Sep 11, 1998·Nature·D C WeinsteinA Hemmati-Brivanlou
Mar 30, 1999·Mechanisms of Development·Y ChenT Pieler
May 8, 1995·Quality in Health Care : QHC·J S Walker, M Wilson

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Citations

Feb 24, 2015·Developmental Biology·Laura J A Hardwick, Anna Philpott
Aug 23, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Pan P LiH Benjamin Peng
Sep 9, 2017·Journal of Molecular Biology·John S KhamoKai Zhang

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