Oncogenic Ras-transformed human fibroblasts exhibit differential changes in contraction and migration in 3D collagen matrices.

Experimental Cell Research
Gustavo C MenezesF Grinnell

Abstract

Tractional force exerted by tissue cells in 3D collagen matrices can be utilized for matrix remodeling or cell migration. The interrelationship between these motile processes is not well understood. The current studies were carried out to test the consequences of oncogenic Ras (H-Ras(V12)) transformation on human fibroblast contraction and migration in 3D collagen matrices. Beginning with hTERT-immortalized cells, we prepared fibroblasts stably transformed with E6/E7 and with the combination HPV16 E6/E7 and H-Ras(V12). Oncogenic Ras-transformed cells lost contact inhibition of cell growth, formed colonies in soft agar and were unable to make adherens junctions. We observed no changes in the extent or growth factor dependence of collagen matrix contraction (floating or stress-relaxation) by oncogenic Ras-transformed cells. On the other hand, transformed cells in nested collagen matrices lost not only growth factor selectivity, but also cell-matrix density-dependent inhibition of migration. These findings demonstrate differential regulation of collagen matrix contraction and cell migration in 3D collagen matrices.

References

Mar 1, 1991·Molecular and Cellular Biology·G G HicksM Mowat
Aug 1, 1983·Journal of Cellular Physiology·D J Buttle, H P Ehrlich
Feb 1, 1994·The Journal of Cell Biology·F Grinnell
Nov 25, 1997·Biophysical Journal·G K RagsdaleK Luby-Phelps
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·N A GloushankovaI M Gelfand
Sep 9, 1999·The Journal of Cell Biology·D A ChereshR L Klemke
Feb 2, 2000·Nature Genetics·J ZuberR Schäfer
Oct 12, 2000·Biochemical and Biophysical Research Communications·M YanaseI Ogata
Oct 29, 2000·Biophysical Journal·T WakatsukiE L Elson
May 4, 2002·Nature Reviews. Molecular Cell Biology·James J TomasekRobert A Brown
Feb 18, 2003·Molecular Biology of the Cell·Frederick GrinnellGabriella Skuta
May 6, 2003·The Journal of Biological Chemistry·Delphine JavelaudAlain Mauviel
May 29, 2003·Biophysical Journal·David I ShreiberRobert T Tranquillo
Mar 17, 2004·Seminars in Cancer Biology·Paul M Campbell, Channing J Der
Mar 24, 2004·Current Opinion in Cell Biology·Peter Friedl
May 18, 2004·Cell Motility and the Cytoskeleton·Ravi K Sawhney, Jonathon Howard
Jul 9, 2004·Molecular Biology of the Cell·B HinzJ-J Meister
Sep 7, 2004·The International Journal of Developmental Biology·Alexis DesmoulièreGiulio Gabbiani
Nov 2, 2004·Nature Reviews. Cancer·Margareta M Mueller, Norbert E Fusenig
Jun 23, 2005·Seminars in Cancer Biology·Dorothy A Beacham, Edna Cukierman
Aug 16, 2005·Current Opinion in Cell Biology·Margit A HuberHartmut Beug
Aug 23, 2005·Current Opinion in Cell Biology·Sharona Even-Ram, Kenneth M Yamada
Oct 4, 2005·Biophysical Journal·S ParkC K Shih
Nov 22, 2005·Oncogene·Jason D Arroyo, William C Hahn
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Jean Paul Thiery, Jonathan P Sleeman
Jan 11, 2007·Journal of Cellular Biochemistry·Derek C RadiskyMina J Bissell
Jan 16, 2007·FEBS Letters·T Y El SayeghC A McCulloch
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sangmyung RheeFrederick Grinnell
May 16, 2007·Current Rheumatology Reports·David J AbrahamThomas Krieg
May 17, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shahram AarabiGeoffrey C Gurtner

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Citations

Jul 7, 2009·Annual Review of Cell and Developmental Biology·Frederick Grinnell, W Matthew Petroll
Sep 12, 2009·Experimental & Molecular Medicine·Sangmyung Rhee
Jun 30, 2010·In Vitro Cellular & Developmental Biology. Animal·Muthulekha SwamydasDidier Dréau
Sep 25, 2015·The Journal of Investigative Dermatology·Jie ChaoMark A Carlson
Mar 27, 2012·Journal of Biomedical Materials Research. Part a·Alvaro Muñoz NovalMiguel Manso Silván
Oct 9, 2013·International Journal of Biomaterials·Mei ZhangAngela M Hong
May 15, 2013·Experimental Cell Research·Bruno da Rocha-Azevedo, Frederick Grinnell

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