Oncogenic transformation of normal breast epithelial cells co-cultured with cancer cells

Cell Cycle
Song LiChenguang Wang

Abstract

The heterogeneity in human breast cancer poses a challenge for effective treatment. Better understanding of tumor initiation and development will help to resolve this problem. Current models explaining intratumoral diversity include cancer stem cells, clonal evolution and cancer cell dedifferentiation and reprogramming. Herein, a new model, cancer transmission, is proposed to explain cancer heterogeneity. We found breast cancer cells (MCF10A.NeuT) were capable of transforming normal mammary epithelial cells (MCF10A). The transformed cells exhibited cancerous properties including enhanced proliferation and migration, loss of apical-basal polarity and depolarized acini structure associated with epithelial-mesenchymal transition (EMT). The transformed MCF10A cells displayed distinct EMT characteristics compared to parental cells. We further showed that cancer cell-secreted factors were sufficient to induce cancerous transformation of normal cells. Furthermore, transformed cells were resistant to radiation treatment, providing new insights into mechanisms underlying therapeutic resistance.

References

Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·R A LueA H Chishti
Feb 13, 2001·The Journal of Clinical Investigation·A S Baldwin
Nov 9, 2001·Annual Review of Genetics·U TepassR Fehon
Dec 4, 2002·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·E IoachimN Pavlidis
Jun 12, 2003·Methods : a Companion to Methods in Enzymology·Jayanta DebnathJoan S Brugge
Apr 1, 2004·British Journal of Cancer·H WillersS N Powell
Jul 21, 2004·Cancer Cell·Minna AllinenKornelia Polyak
Sep 8, 2005·Seminars in Cell & Developmental Biology·Håkan AxelsonSven Påhlman
Sep 5, 2006·Cancer Research·Jason J Christiansen, Ayyappan K Rajasekaran
Dec 13, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Antonio C WolffUNKNOWN College of American Pathologists
Feb 14, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yuko Mimori-KiyosueShoichiro Tsukita
Feb 24, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lyndsay N HarrisEric P Winer
Apr 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiaoming JuRichard G Pestell
Nov 3, 2007·The Journal of Clinical Investigation·Kornelia Polyak
Feb 21, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Marianne KyndiUNKNOWN Danish Breast Cancer Cooperative Group
Apr 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christine L ChafferRobert A Weinberg
Jan 20, 2012·Nature·Mel Greaves, Carlo C Maley
Mar 13, 2012·Seminars in Cancer Biology·Neha TiwariGerhard Christofori
Jul 18, 2012·Laboratory Investigation; a Journal of Technical Methods and Pathology·Steven J PottsHolger Lange
Sep 25, 2012·Cancer Research·Charles Swanton
Oct 25, 2012·Annual Review of Pathology·Vanessa AlmendroKornelia Polyak
Aug 31, 2013·Journal of Cancer Research and Clinical Oncology·Nadire DuruJian Jian Li
Mar 13, 2014·Cell Stem Cell·Antonija Kreso, John E Dick
Jan 13, 2015·Cell Stem Cell·Michalina Janiszewska, Kornelia Polyak
Aug 8, 2015·Nature Medicine·Ash A AlizadehJessica Zucman-Rossi
Sep 24, 2015·The Biochemical Journal·Penny A Jeggo, Markus Löbrich
Feb 16, 2016·Advanced Drug Delivery Reviews·Mechthild KrauseMichael Baumann
Feb 26, 2016·Molecular Cancer·Silvia Juliana Serrano-GomezSuresh K Alahari
Sep 20, 2016·Cancer Treatment Reviews·Konstantinos TryfonidisMartine Piccart

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Citations

Dec 28, 2019·Materials·Bhargav D Mansuriya, Zeynep Altintas
Dec 29, 2020·Molecular and Cellular Endocrinology·Qiong WangJie Zhou

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Methods Mentioned

BETA
flow cytometry
Protein Array
Antibody Array
chromosomal aberrations
fluorescence-activated cell sorting
light microscopy

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