Oncology-Inspired Treatment Options for COVID-19.

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
Nagavarakishore PillarsettyThomas Reiner

Abstract

CR3022 is a human antibody that binds to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we explore the use of CR3022 as a molecularly targeted radiotherapeutic. Methods: CR3022 was labeled with 131I and purified, yielding 131I-CR3022. Using a magnetic bead assay and a recombinant SARS-CoV-2 spike protein fragment, we tested binding of 131I-CR3022 in the presence and absence of CR3022. Results: We conjugated the antibody CR3022 with a purity of more than 98% and a specific activity of more than 292 MBq/mg. Using a bead-based assay, we confirmed that binding of 131I-CR3022 is selective and is significantly reduced in the presence of unlabeled antibody (3.14% ± 0.14% specific uptake and 0.10% ± 0.01% specific uptake, respectively; P < 0.0001). Conclusion: Our results confirm the potential of CR3022 as a molecularly targeted probe for SARS-CoV-2. A labeled version of CR3022 could potentially be used for Auger radiotherapy or noninvasive imaging.

References

Sep 2, 2005·Nature·Gerald Willimsky, Thomas Blankenstein
Sep 21, 2005·Biochemical and Biophysical Research Communications·David DingliStephen J Russell
Mar 23, 2006·Annual Review of Immunology·Zeev Pancer, Max D Cooper
Dec 1, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Kim KramerNai-Kong V Cheung
Jan 12, 2011·Annual Review of Immunology·Matthew D VeselyMark J Smyth
Nov 20, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Philip J Blower
Jul 9, 2016·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Clemens KratochwilAlfred Morgenstern
Jan 12, 2017·The New England Journal of Medicine·Jonathan StrosbergUNKNOWN NETTER-1 Trial Investigators
May 4, 2017·AJR. American Journal of Roentgenology·Hossein Jadvar
Mar 3, 2018·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Sophie PotyJason S Lewis
Sep 5, 2018·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Kelly J McKelveyConnie I Diakos
Sep 21, 2018·Frontiers in Pharmacology·Bas M BavelaarKatherine A Vallis
Oct 30, 2019·EJNMMI Radiopharmacy and Chemistry·Anthony KuRaymond M Reilly
Feb 19, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Giacomo PirovanoThomas Reiner
Apr 14, 2020·The Journal of Infection·Qing YeJianhua Mao
Mar 5, 2020·BioRxiv : the Preprint Server for Biology·Chuang LiuLei Liu

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Citations

Feb 23, 2021·ACS Pharmacology & Translational Science·Roger M Pallares, Rebecca J Abergel
Jun 1, 2021·Nuclear Medicine and Biology·Giacomo PirovanoNaga Vara Kishore Pillarsetty
Jan 30, 2021·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Ekaterina Dadachova
Jul 12, 2021·Seminars in Nuclear Medicine·Lara AntoniosVasken Dilsizian
Apr 25, 2021·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Nagavarakishore PillarsettyThomas Reiner

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