Oncostatin M Promotes Osteoblastic Differentiation of Human Vascular Smooth Muscle Cells Through JAK3-STAT3 Pathway

Journal of Cellular Biochemistry
Yoshinori KakutaniMasaaki Inaba

Abstract

Vascular calcification is a clinically significant component of atherosclerosis and arises from chronic vascular inflammation. Oncostatin M (OSM) derived from plaque macrophages may contribute to the development of atherosclerotic calcification. Here, we investigated the stimulatory effects of OSM on osteoblastic differentiation of human vascular smooth muscle cells (HVSMC) derived from various arteries including umbilical artery, aorta, and coronary artery and its signaling pathway. Osteoblastic differentiation was induced by exposure of HVSMC to osteogenic differentiation medium (ODM) (10% fetal bovine serum, 0.1 μM dexamethasone, 10 mM β-glycerophosphate and 50 μg/ml ascorbic acid 2-phosphate in Dulbecco's modified Eagle's medium [DMEM]). OSM significantly increased alkaline phosphate (ALP) activity and matrix mineralization in HVSMC from all sources. Osteoblast marker genes such as ALP and Runx2 were also up-regulated by OSM in these cells. OSM treatment induced activation of STAT3 in HVSMC from umbilical artery as evidenced by immunoblot. Moreover, not only a JAK3 inhibitor, WHI-P154, but also knockdown of JAK3 by siRNA prevented the OSM-induced ALP activity and matrix mineralization in umbilical artery HVSMC. On the other...Continue Reading

References

May 1, 1994·The Journal of Clinical Investigation·K E WatsonL L Demer
Oct 5, 2002·Circulation Research·Farhad ParhamiLinda L Demer
May 30, 2003·The Biochemical Journal·Peter C HeinrichFred Schaper
Jun 18, 2003·Reviews of Physiology, Biochemistry and Pharmacology·M Tanaka, A Miyajima
Jul 13, 2005·Circulation·Rozemarijn VliegenthartJacqueline C M Witteman
Aug 4, 2005·American Journal of Epidemiology·Michael J LaMonteMilton Z Nichaman
Sep 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuliya VengrenyukSheldon Weinbaum
Jun 18, 2011·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Thorsten DerlinSusanne Klutmann
Dec 2, 2011·Methods in Molecular Biology·Isabel R OrrissTimothy R Arnett
Jul 31, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kasturi GaneshSashwati Roy
Oct 18, 2012·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Myra S CockerRob S B Beanlands
Sep 27, 2013·JAK-STAT·Jiliang Li

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Citations

Dec 15, 2017·Journal of Atherosclerosis and Thrombosis·Atsushi Shioi, Yuji Ikari
May 6, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Yung-Chun WangShi-You Chen
Dec 26, 2019·The Tohoku Journal of Experimental Medicine·Kenta FujimotoMasaaki Inaba
Jul 16, 2020·Experimental Biology and Medicine·Longfei PanHongyan Tian
Feb 28, 2020·Nutrients·Atsushi ShioiMasanori Emoto
Oct 22, 2020·International Journal of Molecular Sciences·Sandra Feijóo-BandínFrancisca Lago
May 25, 2021·Frontiers in Molecular Biosciences·Ping LinShou-Dong Guo
Aug 26, 2021·Cardiovascular Research·Francesca Bartoli-LeonardElena Aikawa
Sep 23, 2021·Cardiovascular Research·O J WaringM M P C Donners
Oct 15, 2021·BMC Cardiovascular Disorders·Fang-Zheng WangYe-Bo Zhou

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