Oncostatin M stimulates proliferation and functions of mouse fetal liver cells in three-dimensional cultures

Journal of Cellular Physiology
Tomo EhashiNorio Ohshima

Abstract

In order to develop a tissue engineered bioartificial liver (BAL), long-term three-dimensional (3-D) culture of fetal liver cells (FLCs) utilizing porous polymer as a scaffold was performed for up to 1 month. The effects of the basal medium and supplementation with oncostatin M (OSM) on the proliferation and differentiation of mouse FLCs were examined in both 3-D culture and conventional monolayer dish culture. Compared with monolayer culture, cell numbers and hepatic function of FLCs were better maintained by 3-D culture. When two kinds of basal media were tested in this study, Williams' medium E (WE) was superior to minimum essential medium alpha (alphaMEM) in expressing hepatic function of FLCs in both 3-D and monolayer cultures, although higher cell densities were obtained with alphaMEM. OSM potently stimulated both cell growth and metabolic activity, especially in 3-D culture. When WE supplemented with OSM was used for 3-D culture, albumin secretion by FLCs increased dramatically after day 5, and a high level of secretion was maintained until the end of culture. During a period of over 1 month, no decrease of albumin secretion was observed. In conclusion, this 3-D culture method was expected to be one of the realistic atte...Continue Reading

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Citations

Feb 6, 2009·Biotechnology Progress·Eric I NovikMartin L Yarmush
Dec 16, 2005·Journal of Biomedical Materials Research. Part a·Tomo EhashiHirotoshi Miyoshi
Dec 12, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mona Dvir-GinzbergSmadar Cohen

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