One-dimensional diffusion on microtubules of particles coated with cytoplasmic dynein and immunoglobulins

Cell Structure and Function
Z Wang, M P Sheetz

Abstract

We characterized and compared the diffusion of beads coated with proteins such as cytoplasmic dynein, alpha-casein, and some immunoglobulins on microtubules. Such weak binding interactions could be common and convenient for concentrating proteins at the surface of cytoplasmic structures such as microtubules. In studying the motile behavior of anionic latex beads coated with limiting dilutions of cytoplasmic dynein, we observed that in addition to active movement, 20-50% of the beads moved back and forth in a random manner. The random movement was inhibited by depletion of ATP or addition of ADP or AMP-PNP. Mean-square-displacement analysis showed that the movement is a one-dimensional diffusion along the microtubule axis with a diffusion coefficient of 2.16 x 10(-10) cm2/sec. Histogram analysis of off-axis movements suggested that approximately 60% of the diffusing beads followed the path of a single microtubule protofilament. Beads coated with proteins such as alpha-casein or a monoclonal immunoglobulin were also observed to diffuse on microtubules with a similar diffusion coefficient to cytoplasmic dynein. However, alpha-casein or immunoglobulin-bead diffusion was not ATP dependent and did not follow the paths of single proto...Continue Reading

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Citations

Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·M Yusuf AliDavid M Warshaw
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Annette GranéliEric C Greene
Mar 13, 2012·Annual Review of Physical Chemistry·Praveen D ChowdaryBianxiao Cui
May 23, 2014·Molecular Biology of the Cell·Virupakshi Soppina, Kristen J Verhey
Oct 1, 2014·Nature Cell Biology·Takayuki TorisawaKen'ya Furuta
Jan 19, 2008·Science·Ram DixitErika L F Holzbaur
Jan 19, 2008·Cellular and Molecular Life Sciences : CMLS·L A Amos
Mar 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·M Yusuf AliKathleen M Trybus
Sep 29, 2012·The Journal of Biological Chemistry·Maike H HinrichsTim Scholz
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Mar 29, 2000·Biophysical Journal·Z Wang, M P Sheetz
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May 18, 2011·Biophysical Journal·Juan Daniel Díaz-ValenciaJennifer L Ross

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