One-dimensional thermotropic dilatation area of lipid headgroups within lamellar lipid/DNA complexes

Langmuir : the ACS Journal of Surfaces and Colloids
Giulio CaraccioloRuggero Caminiti

Abstract

Using simultaneous synchrotron small- and wide-angle X-ray diffraction (SWAXD), we investigated the thermotropic behavior of a cationic lipid mixture of DOTAP-DOPC (1,2-dioleoyl-3-trimethylammonium-propane-dioleoylphosphatidylcholine) liposomes complexed with calf thymus DNA. The DOTAP-DOPC/DNA complex reacts to temperature change by a bilayer compression normal to its surface and an expansion of the DNA in the plane of the rod lattice. By applying two independent recently developed models, we show here for the first time that the thermotropic dilatation area of lipid headgroups within the complexes is not isotropic but occurs parallel to the 1D DNA lattice (i.e., along the direction perpendicular to the DNA axis). Our results shed light on the role of spatial dimensionality in the DNA packing density within lamellar lipoplexes and provide experimental evidence that the interaction between DNA molecules confined between lipid bilayers can be regarded as a 1D problem.

References

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Citations

Nov 3, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Giulio CaraccioloAldo Laganà
Jul 31, 2009·Chemical Communications : Chem Comm·Santanu Bhattacharya, Avinash Bajaj
Jun 20, 2014·Physical Chemistry Chemical Physics : PCCP·Fu-Gen WuZhi-Wu Yu
Dec 3, 2014·Colloids and Surfaces. B, Biointerfaces·Giulio CaraccioloRoberto Coppola
Aug 9, 2008·Dalton Transactions : an International Journal of Inorganic Chemistry·Giampaolo BaroneVincenzo Turco Liveri
Sep 17, 2010·Colloids and Surfaces. B, Biointerfaces·Heinz AmenitschAldo Laganà
Jul 31, 2007·Biochimica Et Biophysica Acta·Giulio CaraccioloHeinz Amenitsch
Jun 22, 2016·Soft Matter·Christopher JanichChristian Wölk
May 14, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Cristina MarchiniGiulio Caracciolo
Sep 19, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Daniela PozziGiulio Caracciolo
Jan 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ying LuMing Li
Aug 1, 2007·Journal of the American Chemical Society·Giulio CaraccioloHeinz Amenitsch
Mar 8, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Giulio CaraccioloAugusto Amici

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