One Hundred False-Positive Amphetamine Specimens Characterized by Liquid Chromatography Time-of-Flight Mass Spectrometry

Journal of Analytical Toxicology
Stephanie J MarinKamisha L Johnson-Davis

Abstract

Some amphetamine (AMP) and ecstacy (MDMA) urine immunoassay (IA) kits are prone to false-positive results due to poor specificity of the antibody. We employed two techniques, high-resolution mass spectrometry (HRMS) and an in silico structure search, to identify compounds likely to cause false-positive results. Hundred false-positive IA specimens for AMP and/or MDMA were analyzed by an Agilent 6230 time-of-flight (TOF) mass spectrometer. Separately, SciFinder (Chemical Abstracts) was used as an in silico structure search to generate a library of compounds that are known to cross-react with AMP/MDMA IAs. Chemical formulas and exact masses of 145 structures were then compared against masses identified by TOF. Compounds known to have cross-reactivity with the IAs were identified in the structure-based search. The chemical formulas and exact masses of 145 structures (of 20 chemical formulas) were compared against masses identified by TOF. Urine analysis by HRMS correlates accurate mass with chemical formulae, but provides little information regarding compound structure. Structural data of targeted antigens can be utilized to correlate HRMS-derived chemical formulas with structural analogs.

References

Dec 31, 2010·Journal of Medical Toxicology : Official Journal of the American College of Medical Toxicology·Erica R CaseyMichael E Mullins
Mar 29, 2011·Journal of Analytical Toxicology·Shawn P VorceJoseph Magluilo
Apr 14, 2012·Pharmacotherapy·Lindsay B Curtin, Michael J Cawley
Jul 18, 2012·Journal of Analytical Toxicology·Stephanie J MarinGwendolyn A McMillin
Oct 16, 2012·Pharmacotherapy·Anne Z DePriestDavid L Black
Jul 6, 2014·Journal of Analytical Toxicology·Alec SaitmanRobert L Fitzgerald
Sep 23, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Li LiuKenichi Tamama

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Citations

Dec 17, 2015·Journal of Analytical Toxicology·Kamisha L Johnson-DavisJonathan R Genzen
Aug 24, 2016·Journal of Analytical Toxicology·Roger L BertholfGary M Reisfield
Sep 19, 2019·The Clinical Biochemist. Reviews·Darren R Allen, Brett C McWhinney
Dec 12, 2018·Attention Deficit and Hyperactivity Disorders·Christina Mohr Jensen, Torben Breindahl

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