PMID: 699044Sep 1, 1978Paper

One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell

Cell
M YamaizumiY Okada

Abstract

Erythrocyte ghosts containing a known number of molecules of purified fragment A of diphtheria toxin with a constant amount of FITC-BSA as a fluorescence marker were prepared by dialyzing a mixture of erythrocytes and these substances against hypotonic solution. These substances were then introduced into diphtheria toxin-resistant mouse L cells by virus-mediated cell fusion of the cells with the ghosts, and mononuclear recipients that has fused with only one erythrocyte ghost were separated in a flourescence-activated cell sorter (FACS) on the basis of their cell size and fluorescence intensity. After separation, the viability of cells containing known numbers of fragment A was examined by measuring colony-forming ability. The results demonstrated that a single molecule of fragment A was sufficient to kill a cell. This fact was confirmed by introduction into cells of fragment A from an immunologically related mutant toxin, CRM 176 (fragment A176); this has a completely functional fragment B region, but in cell extracts, the enzymic activity of its fragment A is about 10 fold less than that of wild toxin. The cytotoxicity of CRM 176 is about two hundredths of that of the wild-type (Uchida, Pappenheimer and Greany, 1973). As expe...Continue Reading

References

Jan 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E MekadaY Okada
Mar 1, 1976·Scientific American·L A HerzenbergL A Herzenberg
Sep 1, 1972·Immunochemistry·A M PappenheimerA A Harper
Nov 1, 1966·Experimental Cell Research·Y Okada, F Murayama
Jun 1, 1968·The Journal of Experimental Medicine·A M Pappenheimer, R Brown
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·G M IhlerF W Schnure
Sep 1, 1971·Nature: New Biology·T UchidaA M Pappenheimer
Jun 1, 1969·Journal of Bacteriology·J L Duncan, N B Groman
Mar 1, 1957·Proceedings of the Society for Experimental Biology and Medicine·J FOGH, R O LUND

❮ Previous
Next ❯

Citations

Sep 2, 1998·International Journal of Cancer. Journal International Du Cancer·L A ChandlerL L Houston
May 16, 2000·The Journal of Gene Medicine·M O ImhofN Mermod
Sep 1, 1994·Protein Science : a Publication of the Protein Society·M J Bennett, D Eisenberg
Dec 24, 2005·Journal of Neurocytology·Huaiyang ChenQizhi Gong
Oct 12, 2005·Plant Molecular Biology·Shigeru Iida, Rie Terada
Mar 25, 2011·Transgenic Research·Michiko SekineHiromichi Yonekawa
Jul 31, 2013·Current Hematologic Malignancy Reports·Robert J Kreitman
Sep 9, 1982·Biochimica Et Biophysica Acta·D P McIntosh, T D Heath
Nov 23, 1983·Biochimica Et Biophysica Acta·V K Jansons, E A Panzner
Dec 16, 1980·Biochimica Et Biophysica Acta·J W MannhalterR J Collier
Sep 1, 1992·Developmental Biology·V A DzelzkalnsM E Nasrallah
Jan 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·G S HarrisonI Castillo
Jan 1, 1993·Molecular Aspects of Medicine·J Harris, K Sikora
Jan 1, 1994·Molecular Aspects of Medicine·G MenestrinaC Montecucco
Jan 1, 1983·Pharmacology & Therapeutics·D C Edwards
Jan 1, 1984·Pharmacology & Therapeutics·K C Zoon, H Arnheiter
Sep 1, 1994·Pharmacology & Therapeutics·V Ghetie, E Vitetta
Jul 22, 1982·Biochimica Et Biophysica Acta·J Tsuzuki, H C Wu
Jan 1, 1988·Trends in Genetics : TIG·R S Beddington
Mar 26, 1992·Biochimica Et Biophysica Acta·E London
Apr 13, 1995·Biochimica Et Biophysica Acta·N Higashi, J Sunamoto
Aug 1, 1993·Trends in Microbiology·G SchiavoC Montecucco
Apr 7, 2004·Advanced Drug Delivery Reviews·Véronique Cabiaux
Apr 15, 2004·Current Opinion in Biotechnology·Shigeru Iida, Rie Terada
Nov 25, 2004·Progress in Biophysics and Molecular Biology·Michael W Parker, Susanne C Feil
Sep 30, 1986·Biochemical and Biophysical Research Communications·R BasosiC S Lai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.