One month of hyperglycemia alters spectral responses of the zebrafish photopic electroretinogram

Disease Models & Mechanisms
Zaid TanvirVictoria P Connaughton

Abstract

Prolonged hyperglycemia can alter retinal function, ultimately resulting in blindness. Adult zebrafish adults exposed to alternating conditions of 2% glucose/0% glucose display a 3× increase in blood sugar levels. After 4 weeks of treatment, electroretinograms (ERGs) were recorded from isolated, perfused, in vitro eyecups. Control animals were exposed to alternating 2% mannitol/0% mannitol (osmotic control) or to alternating water (0% glucose/0% glucose; handling control). Two types of ERGs were recorded: (1) native ERGs measured using white-light stimuli and medium without synaptic blockers; and (2) spectral ERGs measured with an AMPA/kainate receptor antagonist, isolating photoreceptor-to-ON-bipolar-cell synapses, and a spectral protocol that separated red (R), green (G), blue (B) and UV cone signals. Retinas were evaluated for changes in layer thickness and for the inflammatory markers GFAP and Nf-κB (RelA or p65). In native ERGs, hyperglycemic b- and d-waves were lower in amplitude than the b- and d-waves of mannitol controls. Alteration of waveshape became severe, with b-waves becoming more transient and ERG responses showing more PIII-like (a-wave) characteristics. For spectral ERGs, waveshape appeared similar in all trea...Continue Reading

References

Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J RobinsonJ E Dowling
Mar 31, 1998·Diabetes·M MizutaniM Lorenzi
Oct 21, 1998·Electroencephalography and Clinical Neurophysiology·V ParisiM G Bucci
Dec 5, 1998·Visual Neuroscience·A HughesW F Patterson
Aug 31, 1999·Survey of Ophthalmology·R Tzekov, G B Arden
Sep 24, 1999·Mechanisms of Development·F ArgentonM Bortolussi
Nov 1, 1960·Stain Technology·K C RICHARDSONE H FINKE
Feb 27, 2004·Investigative Ophthalmology & Visual Science·Heather A Hancock, Timothy W Kraft
May 13, 2004·Critical Care Nursing Quarterly·Richard A Guthrie, Diana W Guthrie
Aug 25, 2004·Investigative Ophthalmology & Visual Science·Pamela M MartinSylvia B Smith
Sep 29, 2004·Investigative Ophthalmology & Visual Science·Kristian KlempHenrik Lund-Andersen
Dec 30, 2004·Investigative Ophthalmology & Visual Science·Chiara GerhardingerPaul Grosu
Jun 13, 2006·American Journal of Ophthalmology·Douglas J RheeKaren S Nipper
Jun 17, 2006·Documenta Ophthalmologica. Advances in Ophthalmology·Jin XuBing Chen
Jun 27, 2006·Investigative Ophthalmology & Visual Science·Joanna A PhippsAlgis J Vingrys
Aug 10, 2006·Japanese Journal of Ophthalmology·Junya KizawaDaijiro Kurosaka
Oct 27, 2006·Investigative Ophthalmology & Visual Science·David J RamseyHaohua Qian
Jan 24, 2007·Neurobiology of Disease·A R SantiagoA F Ambrósio
Feb 1, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·C J LaytonN N Osborne
Apr 18, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Xiuming JinJia Qu
Jul 21, 2007·Experimental Diabetes Research·Renu A Kowluru, Pooi-See Chan
Sep 28, 2007·Investigative Ophthalmology & Visual Science·Mahesh NagarajuVittorio Porciatti
Oct 17, 2007·BMC Developmental Biology·Yolanda AlvarezBreandan N Kennedy
Mar 15, 2008·Seminars in Immunopathology·Anthony P Adamis, Adrienne J Berman
Apr 19, 2008·Investigative Ophthalmology & Visual Science·Kenichi KohzakiBang V Bui
Feb 11, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Mary D Kinkel, Victoria E Prince
Sep 3, 2009·Visual Neuroscience·Ralph F Nelson, Nirmish Singla
Oct 10, 2009·Pharmacological Reports : PR·Sandip Patel, Dev Santani
Feb 10, 2010·Disease Models & Mechanisms·Yolanda AlvarezBreandán N Kennedy

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Citations

Feb 28, 2020·Investigative Ophthalmology & Visual Science·Zaheer AliRobin A Kimmel
Oct 7, 2020·Visual Neuroscience·Silke BeckerFrans Vinberg
Nov 26, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Olivia ThibaultLene H Petersen
May 13, 2019·American Journal of Ophthalmology·Richard F Spaide
Apr 4, 2021·Cells·Salvatore L StellaStephanie L Grillo
Dec 2, 2021·Journal of Cell Science·Kayla F Titialii-Torres, Ann C Morris

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Methods Mentioned

BETA
protein assay

Software Mentioned

SigmaPlot
Origin
QCapture Plus
pCLAMP
GraphPad
SPSS
ImageJ
Prism

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