One-pot bio-synthesis: N-acetyl-D-neuraminic acid production by a powerful engineered whole-cell catalyst.

Scientific Reports
Fei TaoPing Xu

Abstract

Whole cell biocatalysis is an important tool for pharmaceutical intermediates synthesis, although it is hindered by some shortcomings, such as high cost and toxicity of inducer, mass transfer resistance caused by cell membrane and side reactions. Whole-cell catalysis using N-acetyl-d-glucosamine 2-epimerase (EC 5.1.3.8) and N-acetyl-d-neuraminic acid (Neu5Ac) aldolase (EC 4.1.3.3) is a promising approach for the production of Neu5Ac, a potential precursor of many anti-viral drugs. A powerful catalyst was developed by packaging the enzymes in an engineered bacterium and using a safe temperature-induced vector. Since the mass transfer resistance and the side reactions were substantially reduced, a high Neu5Ac amount (191 mM) was achieved. An efficient method was also presented, which allows one-pot synthesis of Neu5Ac with a safe and economic manner. The results highlight the promise of large-scale Neu5Ac synthesis and point at a potential of our approach as a general strategy to improve whole-cell biocatalysis.

References

Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·P A SrereK Mosbach
Apr 1, 1997·Enzyme and Microbial Technology·M MahmoudianM J Dawson
Sep 5, 1997·Science·F R BlattnerY Shao
Mar 6, 1998·Trends in Biotechnology·G Hannig, S C Makrides
Jan 20, 1999·Cellular and Molecular Life Sciences : CMLS·C Traving, R Schauer
Nov 5, 1999·Protein Expression and Purification·S C Makrides
Mar 11, 2000·Lancet·L V GubarevaF G Hayden
Apr 25, 2000·Angewandte Chemie·T D Machajewski, C H Wong
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Nov 25, 2000·Biochimica Et Biophysica Acta·M le MaireJ V Moller
Jun 26, 2001·Glycoconjugate Journal·R Schauer
Aug 14, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·J L DingB Ho
Feb 1, 1964·Biochemistry·E J MCGUIRE, S B BINKLEY
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·T B CauseyL O Ingram
Apr 28, 2004·Journal of Industrial Microbiology & Biotechnology·Shu-Jen Chiang
Apr 7, 2005·Current Opinion in Chemical Biology·Takeru IshigeSakayu Shimizu
Jan 16, 2007·Applied Microbiology and Biotechnology·Rachel Ruizhen Chen
Dec 1, 2007·Nature Reviews. Drug Discovery·Mark von Itzstein
Mar 6, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Naoki KawaiSeizaburo Kashiwagi
Jun 11, 2009·Nature Biotechnology·Venkataramanan SoundararajanRam Sasisekharan
Mar 29, 2011·Applied and Environmental Microbiology·Xiaoman XuPing Xu

❮ Previous
Next ❯

Citations

Feb 20, 2013·Applied Microbiology and Biotechnology·Bai-Xue LinYong Tao
Sep 29, 2012·Metabolic Engineering·Junhua KangQingsheng Qi
Jan 27, 2015·Applied Microbiology and Biotechnology·Fang ChenBai-Xue Lin
May 15, 2016·Enzyme and Microbial Technology·Ludwig KlermundKathrin Castiglione
Mar 26, 2015·Protein Expression and Purification·Ludwig KlermundKathrin Castiglione
Aug 24, 2017·Chemistry : a European Journal·Xun LvXuebing Li
Jun 12, 2019·PloS One·Man Kumari GurungInger Lin U Ræder
Oct 20, 2015·Applied Microbiology and Biotechnology·Bai-Xue LinYong Tao
Jun 15, 2017·Microbial Cell Factories·Baixue Lin, Yong Tao
Dec 17, 2020·Chemical Society Reviews·Sjoerd Slagman, Wolf-Dieter Fessner
Dec 8, 2020·Biotechnology Advances·Haiquan YangXianzhong Chen
Jul 22, 2021·Biotechnology and Bioengineering·Sabine SchelchBernd Nidetzky

❮ Previous
Next ❯

Methods Mentioned

BETA
phosphotransferase
restriction digest
PCR
protein assay

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.