One-pot synthesis of dopamine dithiocarbamate functionalized gold nanoparticles for quantitative analysis of small molecules and phosphopeptides in SALDI- and MALDI-MS

The Analyst
Suresh Kumar Kailasa, Hui-Fen Wu

Abstract

The sensitivity and efficiency of SALDI-MS or MALDI-MS is mainly dependent on the nature of matrix. A novel approach is proposed for one-pot synthesis of dopamine dithiocarbamate-functionalized gold nanoparticles (DDTC-Au NPs). Their application to quantification of small molecules by surface assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF-MS) and rapid identification of phosphopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is investigated. The synthesized DDTC-Au NPs were characterized by UV-visible and FT-IR spectroscopy, H(1)NMR, SEM and TEM. DDTC-Au NPs offers marked improvement on analyte ionization and effectively suppressed the background noise which leads to clean mass spectra. We also demonstrated the use of DDTC-Au NPs as affinity probes for selective enrichment of phosphopeptides from the solutions of microwave tryptic digested casein proteins. Compared with a conventional matrix, DDTC-Au NPs exhibited a high desorption/ionization efficiency for accurate quantification of small molecules including amino acid (glutathione), drugs (desipramine and enrofloxacin) and peptides (valinomycin and gramicidin D) and successfully utilized ...Continue Reading

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Citations

Jul 25, 2013·Environmental Science and Pollution Research International·Bojidarka Ivanova, Michael Spiteller
Jul 27, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Aytaç GültekinRıdvan Say
Mar 15, 2016·Analytical and Bioanalytical Chemistry·Hani Nasser Abdelhamid, Hui-Fen Wu
Sep 21, 2016·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Ching-Yi WuYu-Chie Chen
Apr 2, 2019·Analytical and Bioanalytical Chemistry·Xi-Yu PanTsung-Rong Kuo
Nov 28, 2019·Nanomedicine·Isabel Ortiz de SolorzanoManuel Arruebo
May 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Judy GopalHui-Fen Wu
Oct 16, 2018·Analytical Methods : Advancing Methods and Applications·Jinlan DongJamie D Mans
Apr 20, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Anuja Shreeram KulkarniKun Qian

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