One pot synthesis of intriguing fluorescent carbon dots for sensing and live cell imaging

Talanta
Jayasmita JanaTarasankar Pal

Abstract

We report a simple one-pot synthesis of highly fluorescent carbon dots (CDs) via modified hydrothermal (MHT) treatment of alkaline solution of dopamine and cysteine. These CDs (λex=320 nm, λem=390 nm, and quantum yield ∼ 5.1%) are of ∼ 2-3 nm in diameter. Further attempt of synthesizing CDs in some common water-miscible solvents ends up the fact that the MHT product from acetone medium is nonfluorescent. However, CDs, produced in aqueous medium, are so stable that they can be dried as a deliverable solid (WCD) without any alteration of fluorescing property if reversibly dispersed in water. Fluorescence of WCD is quenched selectively in acetone. Quenching occurs presumably due to the disruption of radiative recombination along with the hindrance in quantum confinement of the emissive energy traps to the particle surface. Successive quenching of fluorescence of WCD in different acetone concentration admixed in water paves the way to selective acetone sensing (LOD=8.75 × 10(-7) M). The synthesized CDs (in aqueous medium) are cytocompatible and are efficient fluorescent probe for cell imaging. Only living cells are recognized exclusively from fluorescence imaging leaving aside dead cells, while cells are treated with CDs.

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Citations

Nov 16, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Ziwei SunHuihua Qu
Feb 21, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Xiaoxiao PengYunfei Long
Nov 2, 2019·Beilstein Journal of Nanotechnology·Tugrul GunerMustafa M Demir
Feb 14, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Jayasmita JanaTarasankar Pal
Sep 6, 2017·Journal of Nanobiotechnology·Xin YanHuihua Qu
Dec 20, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Junyang DongHao Zhang
Feb 16, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Min TianYong Zhang
Nov 26, 2020·International Journal of Nanomedicine·Yusheng ZhaoYan Zhao
Mar 25, 2017··Gleb B. SukhorukovAlina A. Gvozdyuk

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