One-Pot Synthesis of Phenylglyoxylic Acid from Racemic Mandelic Acids via Cascade Biocatalysis

Journal of Agricultural and Food Chemistry
Cunduo TangYun-Chao Kan

Abstract

Phenylglyoxylic acid (PGA) are key building blocks and widely used to synthesize pharmaceutical intermediates or food additives. However, the existing synthetic methods for PGA generally involve toxic cyanide and complex processes. To explore an alternative method for PGA biosynthesis, we envisaged cascade biocatalysis for the one-pot synthesis of PGA from racemic mandelic acid. A novel mandelate racemase named ArMR showing higher expression level (216.9 U·mL-1 fermentation liquor) was cloned from Agrobacterium radiobacter and identified, and six recombinant Escherichia coli strains were engineered to coexpress three enzymes of mandelate racemase, d-mandelate dehydrogenase and l-lactate dehydrogenase, and transform racemic mandelic acid to PGA. Among them, the recombinant E. coli TCD 04, engineered to coexpress three enzymes of ArMR, LhDMDH, and LhLDH, can transform racemic mandelic acid (100 mM) to PGA with 98% conversion. Taken together, we provide a green approach for one-pot biosynthesis of PGA from racemic mandelic acid.

References

Sep 15, 2010·Biotechnology Advances·Carla C C R de Carvalho
May 12, 2012·Nature·U T BornscheuerK Robins
Jun 27, 2012·Journal of Biomolecular Structure & Dynamics·Jiali Gu, Hongwei Yu
Feb 21, 2018·Journal of Agricultural and Food Chemistry·Cun-Duo TangYun-Chao Kan

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Citations

Oct 14, 2020·Critical Reviews in Biotechnology·Ji-Dong ShenYu-Guo Zheng

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