One-Pot Two-Step Metabolic Labeling of Teichoic Acids and Direct Labeling of Peptidoglycan Reveals Tight Coordination of Both Polymers Inserted into Pneumococcus Cell Wall

ACS Chemical Biology
Julie BonnetC Durmort

Abstract

A method for labeling teichoic acids in the human pathogen Streptococcus pneumoniae has been developed using a one-pot two-step metabolic labeling approach. The essential nutriment choline modified with an azido-group was incorporated and exposed at the cell surface more rapidly than it reacted with the strain promoted azide alkyne cycloaddition (SPAAC) partner also present in the medium. Once at the cell surface on teichoic acids, coupling of the azido group could then occur within 5 min by the bio-orthogonal click reaction with a DIBO-linked fluorophore. This fast and easy method allowed pulse-chase experiments and was combined with another fluorescent labeling approach to compare the insertion of teichoic acids with peptidoglycan synthesis with unprecedented temporal resolution. It has revealed that teichoic acid and peptidoglycan processes are largely concomitant, but teichoic acid insertion persists later at the division site.

References

Jul 1, 1985·Journal of Bacteriology·H Fischer, A Tomasz
Jan 16, 2008·FEMS Microbiology Reviews·Waldemar VollmerMiguel A de Pedro
Nov 10, 2009·Chembiochem : a European Journal of Chemical Biology·Jonathan G SwobodaSuzanne Walker
Oct 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Magda L AtilanoSérgio R Filipe
Jun 21, 2011·Journal of the American Chemical Society·Tania J LupoliDaniel Kahne
Mar 1, 2012·Bioconjugate Chemistry·Remon van GeelWilbert C Boelens
Mar 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Alice EberhardtWaldemar Vollmer
Mar 16, 2013·Trends in Biotechnology·Samir GautamDavid A Spiegel
Sep 13, 2013·Annual Review of Microbiology·Stephanie BrownSuzanne Walker
Jan 5, 2014·Photochemistry and Photobiology·Caishun LiChristopher W Cairo
Mar 1, 2015·FEMS Microbiology Reviews·M Sloan SiegristCarolyn R Bertozzi
Aug 31, 2016·Topics in Current Chemistry·Jan DommerholtFloris L van Delft

❮ Previous
Next ❯

Citations

Nov 21, 2019·Protein Science : a Publication of the Protein Society·Jed F Fisher, Shahriar Mobashery

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.