One protein from two open reading frames: mechanism of a 50 nt translational bypass

The EMBO Journal
A J HerrJohn F Atkins

Abstract

Translating ribosomes bypass a 50 nt coding gap in order to fuse the information found in the two open reading frames (ORFs) of bacteriophage T4 gene 60. This study investigates the underlying mechanism by focusing on the competition between initiation of bypassing and termination at the end of the first ORF. While nearly all ribosomes initiate bypassing, no more than 50% resume translation in the second ORF. Two previously described cis-acting stimulatory signals are critical for favoring initiation of bypassing over termination. Genetic analysis of these signals supports a working model in which the first (a stem-loop structure at the junction between the first ORF and the coding gap) interferes with decoding in the A-site, and the second (a stretch of amino acids in the nascent peptide encoded by the first ORF) destabilizes peptidyl-tRNA-mRNA pairing.

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Citations

Oct 29, 2003·Biochimica Et Biophysica Acta·Michael O'Connor
Jul 16, 2011·The Journal of Biological Chemistry·Jason S SeidmanChristopher S Hayes
Sep 13, 2000·Nucleic Acids Research·B MooreJ F Atkins
Apr 25, 2003·Nucleic Acids Research·Olivier NamyJean-Pierre Rousset
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·J F AtkinsR F Gesteland
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·John F Atkins, Glenn R Björk
Apr 22, 2004·RNA·Michael J TrimbleKelly P Williams
Oct 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jonathan GallantDale Lindsley
Apr 4, 2002·Molecular Microbiology·Olivier NamyJean-Pierre Rousset
May 28, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazushige KurohaToshifumi Inada
Sep 6, 2008·The EMBO Journal·Norma M WillsJohn F Atkins
Aug 12, 2015·Nature Reviews. Genetics·Pavel V BaranovMartina M Yordanova
Jun 25, 2009·BMC Evolutionary Biology·Denice C Bay, Raymond J Turner
Feb 5, 2004·Molecular Cell·Olivier NamyIan Brierley
Sep 24, 2016·Quarterly Reviews of Biophysics·Jin ChenJoseph D Puglisi
Aug 9, 2001·Journal of Molecular Biology·A J HerrJ F Atkins
May 14, 2005·Journal of Molecular Biology·Dale LindsleyAshley Fontelera
Jun 21, 2017·Science Advances·Xabier AgirrezabalaMikel Valle
Jan 11, 2003·The Journal of Biological Chemistry·Pablo de FelipeJeremy D Brown
Jun 6, 2003·Molecular Microbiology·Dale LindsleyGabriel Guarneros
Aug 21, 2007·Biology of the Cell·J Ross Buchan, Ian Stansfield
Jun 12, 2019·Science Advances·M KlimovaM V Rodnina

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