PMID: 11916450Mar 28, 2002Paper

One-step affinity purification of fetuin from fetal bovine serum

Biotechnology and Applied Biochemistry
Simone CartellieriBernd Niemeyer

Abstract

Fetuin is a plasma glycoprotein widely distributed in mammals. It has been used as a model protein for structural analyses and investigations into the biological properties of glycoproteins. A convenient one-step procedure for biospecific isolation of fetuin from fetal bovine serum was developed on the basis of wheatgerm agglutinin (WGA) affinity separation. Two different porous supports, a silica-based material and a polymer-based material, were used for the immobilization of WGA. The prepared WGA adsorbents were characterized and process parameters of the affinity separation of fetuin were investigated and optimized. WGA was immobilized on silica and polymer supports with coupling yields of 99.6 and 99.4% respectively and amounts of coupled ligand of 7.9 and 9.2 mg of WGA/ml respectively. It has been shown that the specific capacities for fetuin were 5.1 mg/ml on WGA-silica, 1.8 mg/ml on WGA-polymer and 4.1 mg/ml on WGA-agarose. All three adsorbents proved to be suitable for the biospecific separation of fetuin. The polymer-based WGA adsorbent was successfully applied in the purification of fetuin from fetal bovine serum in a one-step separation process. The identity and purity of the isolated product was verified by SDS/PAGE...Continue Reading

Citations

May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·N E Labrou
Mar 27, 2013·Chemical Research in Toxicology·Christian BassetClaude Vidaud
Feb 20, 2008·PLoS Pathogens·Didier RaoultPatricia Renesto
Feb 2, 2016·Atherosclerosis·Chisato NaitoTakuya Watanabe
Jul 19, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Heike HelmholzAndreas Prange
Nov 6, 2008·Rapid Communications in Mass Spectrometry : RCM·Benjamin MannMilos V Novotny
Oct 19, 2007·The Journal of Biological Chemistry·Ronald L Mellgren, Xinhua Huang
Sep 29, 2005·Journal of Chromatography. a·Henning RosenfeldAndreas Prange

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