Abstract
Focal full-thickness articular cartilage defects are challenging to repair. The purpose of this study was to find a simple, effective 1-step articular cartilage repair method. Because stem cell niches produce a microenvironment for stem cell self-renewal, proliferation, and differentiation, we integrated in situ bone marrow stem cells with an implanted poly(L-lactic-co-glycolic acid) (PLLGA) scaffold. Marrow stem cells grew and proliferated on cell-free PLLGA scaffolds, which were evaluated by scanning electronic microscopy (SEM) and Cell Counting Kit-8 (Dojindo, Kumamoto, Japan). Twenty-seven rabbits (54 knees) with large cylinder femoral trochlear cartilage defects were created and repaired with microfracture and cell-free PLLGA scaffold implantation (group 1), microfracture (group 2), or cell-free PLLGA scaffold implantation (group 3).Outcomes were evaluated by magnetic resonance imaging, International Cartilage Repair Society scores, histology, and immunohistochemistry. The repair effects were better in group 1 than in groups 2 and 3. In group 1, hyaline-like cartilage formed at week 24. Magnetic resonance imaging showed homogeneous signals as the adjacent normal cartilage. Collagen type II and toluidine blue were stained p...Continue Reading
References
Oct 24, 1998·The Journal of Orthopaedic and Sports Physical Therapy·J A Buckwalter
Aug 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A A WorsterA J Nixon
Nov 2, 2001·Nature·A SpradlingT Kai
Sep 19, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ming PeiLisa E Freed
Dec 31, 2002·Osteoarthritis and Cartilage·Y DausseE Payan
Feb 7, 2003·The Journal of Bone and Joint Surgery. American Volume·U HorasR Schnettler
Dec 23, 2003·Stem Cells·Danièle NoëlChristian Jorgensen
May 18, 2004·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Alberto GobbiKonrad Malinowski
Mar 2, 2005·Developmental Cell·Linheng Li
Aug 19, 2005·Journal of Biomedical Materials Research. Part a·Luciana De FranceschiRoberto Giardino
Sep 6, 2005·The Journal of Bone and Joint Surgery. American Volume·Kai MithoeferRobert G Marx
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Linheng Li, Ting Xie
Jan 18, 2006·Journal of Biomaterials Science. Polymer Edition·Ho Joon ShinJung-Woog Shin
Nov 23, 2006·The Journal of Bone and Joint Surgery. American Volume·Deryk G Jones, Lars Peterson
Mar 30, 2007·Arthritis Research & Therapy·Farida DjouadChristian Jorgensen
Apr 13, 2007·The Knee·W WiduchowskiT Trzaska
Feb 22, 2008·European Cells & Materials·S MunirahG Khang
Feb 26, 2008·International Journal of Oral and Maxillofacial Surgery·C Ferretti
Mar 12, 2008·Blood·Felicia NgVivek Tanavde
Aug 12, 2008·Cells, Tissues, Organs·Anja DrengkKarl-Heinz Frosch
Aug 30, 2008·The American Journal of Sports Medicine·Richard D FerkelRyan M Dopirak
Sep 17, 2008·Biomaterials·Xin ZhangYingfang Ao
Mar 3, 2009·The American Journal of Sports Medicine·Kai MithoeferBert R Mandelbaum
Apr 11, 2009·Implant Dentistry·Muna SoltanJennifer H Choi
Jan 23, 2010·The American Journal of Sports Medicine·Santos Martinez-DiazJoan Carles Monllau
Citations
Jan 29, 2014·Plastic and Reconstructive Surgery·Jeffrey E Janis, Bridget Harrison
May 1, 2013·Regenerative Medicine·Ken YeDamian E Myers
Sep 11, 2014·International Journal of Molecular Sciences·Michal JagielskiGundula Schulze-Tanzil
Nov 30, 2013·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Junjun ShiYingfang Ao
Nov 30, 2013·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·James H P HuiUNKNOWN Asian Cartilage Society
May 11, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jenel M PatrascuChristian Kaps
Apr 9, 2016·Photomedicine and Laser Surgery·Reza FekrazadMostafa Sadeghi Ghuchani
Jan 9, 2013·Expert Opinion on Therapeutic Targets·Robert J MacFarlaneEleftherios Tsiridis
Mar 29, 2016·Tissue Engineering. Part a·Makiko NogamiToshio Nikaido
Aug 25, 2016·Plastic and Reconstructive Surgery·Jeffrey E Janis, Bridget Harrison
Mar 10, 2018·Journal of Tissue Engineering and Regenerative Medicine·Mohammad-Hossein BeigiMohammad-Hossein Nasr-Esfahani
Oct 7, 2014·Macromolecular Bioscience·Nandana BhardwajBiman B Mandal
Apr 12, 2019·Cartilage·Raquel VayasAraceli Delgado
Nov 2, 2018·Archives of Orthopaedic and Trauma Surgery·Sebastian G WalterFrank A Schildberg
Dec 30, 2017·Experimental and Therapeutic Medicine·Hong-Dan FuDai-He Li
May 12, 2018·Genes·Julia ScheinpflugFrank Schulze