PMID: 11924995Apr 2, 2002Paper

One-step concentration of analytes based on dynamic change in pH in capillary zone electrophoresis

Analytical Chemistry
Wei WeiEdward S Yeung

Abstract

A novel strategy for one-step concentration of analytes during capillary electrophoresis (CE) is presented. A short platinum wire was inserted into the 75-microm-i.d. separation capillary. When a high voltage was applied for CE separation, a sharp pH gradient along the capillary was created dynamically by the electrolysis of water in the running buffer. Concentration of a large volume of injected analytes was accomplished by the change in analyte charge due to the dynamic pH gradient. Depending on the polarity of the applied potential and the direction of electroosmotic flow, either anions or cations can be concentrated. Several hundredfold concentration factors were achieved. Fluorescence imaging by a CCD camera was used to monitor 10 cm of the capillary near the platinum wire during the concentration process. The observations are consistent with a sweeping mechanism.

Citations

Apr 2, 2011·Analytical and Bioanalytical Chemistry·Gabriel Loget, Alexander Kuhn
Jul 25, 2003·Journal of Chromatography. a·Philip Britz-McKibbin, Shigeru Terabe
May 31, 2013·Accounts of Chemical Research·Gabriel LogetAlexander Kuhn
Dec 31, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takashi KanetaTotaro Imasaka
Aug 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Fumihiko KitagawaKoji Otsuka
Nov 24, 2007·Journal of Chromatography. a·Steven L SimpsonShigeru Terabe
Feb 4, 2005·Electrophoresis·Xing-Zheng WuKoji Onoda
Jun 18, 2009·Lab on a Chip·Dzmitry HlushkouUlrich Tallarek

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