One-Step Duplex Droplet Digital PCR for WT1 Overexpression.

The Journal of Molecular Diagnostics : JMD
Laurent DewispelaereHakim El Housni

Abstract

With the improvement of treatment methods in acute hematology malignancies, the development of sensitive tools for minimal residual disease assessment has become a priority. The monitoring of WT1 expression level by real-time quantitative PCR has been a standard for minimal residual disease evaluation in acute myeloid leukemia and, since 2009, has been optimized through a European LeukemiaNet effort in an established protocol with well-defined clinical end points. Building on the work of the European LeukemiaNet, this article reports the development of a novel, one-step duplex WT1/ABL1 droplet digital assay for WT1 overexpression detection. This assay provides accurate data with high precision and linearity, even at low-template concentration, while retaining strong correlation with the standardized method and therefore maintaining the framework to analyze the results in the context of acute myeloid leukemia patients.

Citations

Dec 8, 2020·Frontiers in Medicine·Margalida Esteva-SociasAntònia Obrador-Hevia

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