One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst

Scientific Reports
Melanie MindtVolker F Wendisch

Abstract

N-methylated amino acids are found in Nature in various biological compounds. N-methylation of amino acids has been shown to improve pharmacokinetic properties of peptide drugs due to conformational changes, improved proteolytic stability and/or higher lipophilicity. Due to these characteristics N-methylated amino acids received increasing interest by the pharmaceutical industry. Syntheses of N-methylated amino acids by chemical and biocatalytic approaches are known, but often show incomplete stereoselectivity, low yields or expensive co-factor regeneration. So far a one-step fermentative process from sugars has not yet been described. Here, a one-step conversion of sugars and methylamine to the N-methylated amino acid N-methyl-L-alanine was developed. A whole-cell biocatalyst was derived from a pyruvate overproducing C. glutamicum strain by heterologous expression of the N-methyl-L-amino acid dehydrogenase gene from Pseudomonas putida. As proof-of-concept, N-methyl-L-alanine titers of 31.7 g L-1 with a yield of 0.71 g per g glucose were achieved in fed-batch cultivation. The C. glutamicum strain producing this imine reductase enzyme was engineered further to extend this green chemistry route to production of N-methyl-L-alanine...Continue Reading

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Citations

Feb 18, 2020·Biotechnology and Applied Biochemistry·Kerstin SchultenkämperVolker F Wendisch
Oct 18, 2019·Frontiers in Bioengineering and Biotechnology·Kareen H VeldmannVolker F Wendisch
Mar 15, 2020·Biotechnology Journal·Melanie MindtVolker F Wendisch
Nov 27, 2018·Frontiers in Bioengineering and Biotechnology·Melanie MindtVolker F Wendisch
Feb 18, 2021·Cellular and Molecular Life Sciences : CMLS·M D MannielloP Falabella
Jun 12, 2020·Microorganisms·Tatjana WalterVolker F Wendisch
Apr 4, 2019·Metabolic Engineering·Volker F Wendisch
Oct 19, 2021·Frontiers in Bioengineering and Biotechnology·Arthur BurgardtVolker F Wendisch
Dec 17, 2021·Physical Chemistry Chemical Physics : PCCP·Darío Barreiro-LageSergio Díaz-Tendero

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