One-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of tomato torrado virus

Archives of Virology
Marta BudziszewskaAleksandra Obrępalska-Stęplowska

Abstract

'Torrado' disease caused by tomato torrado virus (ToTV) is responsible for considerable losses in tomato production. Therefore, a one-step reverse transcription loop-mediated isothermal amplification protocol for early and fast detection of ToTV isolates has been developed. The RNA extracted from ToTV-infected plants was tested using this protocol with a set of six primers specific for the Vp35 coat protein gene sequence. The amplified products were analyzed using amplification curves, electrophoresis, and direct staining of DNA. The sensitivity of the protocol was tenfold higher than that of conventional RT-PCR. This new protocol is inexpensive, rapid, simple, and very sensitive.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
Sep 11, 2008·Virus Genes·Marta BudziszewskaHenryk Pospieszny
Sep 17, 2010·Applied Biochemistry and Biotechnology·Shijun FuZhiqiang Shen
Nov 25, 2014·Archives of Virology·Przemysław WieczorekAleksandra Obrępalska-Stęplowska
Mar 21, 2015·PloS One·Arnika PrzybylskaAleksandra Obrępalska-Stęplowska
May 10, 2015·Journal of Virological Methods·José Angel Herrera-VásquezInmaculada Ferriol
Jun 7, 2015·Annual Review of Phytopathology·René A A van der VlugtJeremy R Thompson
Apr 1, 2012·Plant Disease·M Verbeek, A M Dullemans
Sep 1, 2010·Plant Disease·S DavinoM Davino
Apr 1, 2010·Plant Disease·C F GambleyB H Hall
Dec 1, 2009·Plant Disease·E VerdinH Lecoq
May 1, 2009·Plant Disease·A Alfaro-FernándezC Jordá
Aug 1, 2007·Plant Disease·A Alfaro-FernándezC Jordá
Oct 1, 2007·Plant Disease·H PospiesznyB Hasiów

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Citations

Jul 26, 2019·Microbiology and Immunology·Kazuya Shirato
Mar 1, 2019·BMJ Global Health·Cassandra Kelly-CirinoMaria D Van Kerkhove
Aug 28, 2021·Cells·Marianna SorokaAnna Rymaszewska

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

LAMP Designer
BLASTN

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