One-step sandwich enzyme immunoassay for human laminin using monoclonal antibodies

Clinica Chimica Acta; International Journal of Clinical Chemistry
N FujimotoA Ooshima

Abstract

A one-step sandwich enzyme immunoassay (one step sandwich EIA) for human serum immunoreactive laminin was set up with a pair of monoclonal antibodies prepared against human placental laminin P1 fragment. The assay was characterized by carrying out two immunoreactions simultaneously, laminin P1 fragment reacting with both a monoclonal antibody as a solid phase and a horseradish peroxidase-labeled monoclonal antibody (Fab') against human laminin P1 fragment as conjugate. Sensitivity of the immunoassay was 0.01 ng/well (0.5 microgram/l), and linearity was obtained between 0.01-20 ng/well (0.5-1,000 micrograms/l). The levels of laminin in sera from normal individuals and patients with liver cirrhosis, hepatocellular carcinoma and primary biliary cirrhosis were 103 +/- 15 micrograms/l, 228 +/- 70 micrograms/l, 341 +/- 163 micrograms/l and 232 +/- 93 micrograms/l, respectively. Protein immunoblotting showed that the serum immunoreactive laminin measured by the assay was a fragment with rel mol mass of 200 kDa.

References

May 31, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·K ObataA Ooshima
Oct 14, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A van ZantenJ H Wilson
Jun 1, 1987·Alcoholism, Clinical and Experimental Research·T NouchiC S Lieber
Apr 15, 1986·Klinische Wochenschrift·B HögemannU Gerlach
Jun 1, 1985·European Journal of Clinical Investigation·O NiemeläJ Risteli
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jan 1, 1980·European Journal of Biochemistry·J RisteliR Timpl
May 1, 1980·Analytical Biochemistry·S I RennardP G Robey

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Citations

Sep 15, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y YokoyaA Ooshima
Mar 9, 2000·Journal of Hepatology·K M WalshA J Morris
May 31, 2012·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V A MitianinaA A Selishcheva

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