One-step simultaneous detection of Ureaplasma parvum and genotypes SV1, SV3 and SV6 from clinical samples using PlexPCR technology

Letters in Applied Microbiology
M S PayneE Mokany

Abstract

Ureaplasma spp. are associated with preterm birth. In recent times, it has become apparent that Ureaplasma parvum, but not Ureaplasma urealyticum, is of most relevance. We recently demonstrated this in Australian pregnant women and using high-resolution melt (HRM) PCR, further showed that U. parvum genotype SV6 was of particular significance. However, our assay was unable to identify multiple genotypes in the same sample, required a separate species-level qPCR for low titre samples and was not ideal for diagnostic laboratories due to the nature of HRM PCR result interpretation. Consequently, our current study developed a novel, one-step PlexPCR assay capable of detecting U. parvum and genotypes SV1, SV3 and SV6 in a single reaction directly from clinical samples. We then validated this using vaginal swab DNA from our Australian cohort of pregnant women. The PlexPCR was highly sensitive, detecting all targets to between 0.4 × 10(-5)  ng DNA (SV3) and 0.4 × 10(-6)  ng DNA (U. parvum, SV1 and SV6). Compared to our HRM PCR, the PlexPCR defined genotype distribution in all seven cases previously reported as 'mixed', and detected another eight cases where multiple genotypes (two) were present in samples previously reported as single ...Continue Reading

References

Jan 1, 1995·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·S HorowitzM Glezerman
Feb 1, 1995·Acta Obstetricia Et Gynecologica Scandinavica·S HorowitzM Glezerman
May 18, 2000·The New England Journal of Medicine·R L GoldenbergW W Andrews
Oct 18, 2000·International Journal of Systematic and Evolutionary Microbiology·F KongG L Gilbert
Nov 18, 2000·Acta Obstetricia Et Gynecologica Scandinavica·M Abele-HornM Kolben
Oct 16, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Dimitris A KafetzisStellios Michalas
Jul 12, 2005·Molecular and Cellular Probes·Jongyoun YiEui-Chong Kim
Oct 15, 2005·Clinical Microbiology Reviews·Ken B WaitesRobert L Schelonka
Jan 5, 2006·Journal of Clinical Microbiology·Soromon KataokaHisanori Minakami
Mar 1, 2008·Acta Obstetricia Et Gynecologica Scandinavica·Dace RezebergaGilbert G G Donders
Oct 14, 2009·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·S GovenderL J Chalkley
Dec 1, 2009·Acta Obstetricia Et Gynecologica Scandinavica·Maria BreugelmansWalter Foulon
Jan 16, 2010·Acta Medica (Hradec Králové)·Marian KacerovskýJindrich Tosner
Jun 18, 2010·Journal of Clinical Microbiology·Li XiaoKen B Waites
Jun 24, 2010·Clinics in Perinatology·Rose M Viscardi
Nov 26, 2010·The Pediatric Infectious Disease Journal·Tae-Jung SungRose M Viscardi
May 9, 2012·Annals of Laboratory Medicine·Seong Jin ChoiAnna Lee
Dec 13, 2012·Clinical Chemistry·Elisa MokanyAlison V Todd
Apr 17, 2013·Current Opinion in Infectious Diseases·Romina CapocciaDavid Baud
Sep 13, 2013·Methods in Molecular Biology·Elisa Mokany, Alison V Todd
Jan 31, 2014·Journal of Clinical Microbiology·Matthew S PayneJohn P Newnham
Dec 17, 2014·Frontiers in Immunology·Matthew S Payne, Sara Bayatibojakhi

❮ Previous
Next ❯

Citations

Nov 14, 2018·Journal of Analytical Methods in Chemistry·Yongjie XuZhishun Lu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
Nadia RodríguezJosé A Rivera
© 2022 Meta ULC. All rights reserved