One-step surface modification of polyurethane using affinity binding peptides for enhanced fouling resistance

Journal of Biomaterials Science. Polymer Edition
Yibing WangPing Wang

Abstract

Affinity binding peptides were examined for surface fabrication of synthetic polymeric materials. Peptides possessing strong binding affinities toward polyurethane (PU) were discovered via biopanning of M13 phage peptide library. The apparent binding constant (K(app)) was as high as 2.68 × 10(9) M(-1) with surface peptide density exceeded 1.8 μg/cm(2). Structural analysis showed that the ideal peptide had a high content (75%) of H-donor amino acid residues, and that intensified hydrogen bond interaction was the key driving force for the highly stable binding of peptides on PU. PU treated with such affinity peptides promises applications as low-fouling materials, as peptides increased its wettability and substantially reduced protein adsorption and cell adhesion. These results demonstrated a facile but highly efficient one-step strategy for surface property modification of polymeric materials for biotechnological applications.

References

May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·J C TillerA M Klibanov
Jan 9, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hossein HosseinkhaniHisatoshi Kobayashi
Apr 24, 2008·Biotechnology Progress·Xiaodong TongPing Wang
May 27, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Hisao MatsunoTakeshi Serizawa
Apr 20, 2011·Colloids and Surfaces. B, Biointerfaces·Pranav Kumar PrabhakarMukesh Doble
Oct 17, 2012·ACS Applied Materials & Interfaces·Liting ZhangPing Wang
Jan 12, 2013·Applied Biochemistry and Biotechnology·Daewon ParkYadong Wang
Apr 4, 2013·Chemical Reviews·Hossein HosseinkhaniDah-Shyong Yu
May 7, 2014·Macromolecular Bioscience·Cristina LluchVirginia Cádiz
Oct 10, 2014·International Journal of Molecular Sciences·Keng-Liang Ou, Hossein Hosseinkhani

❮ Previous
Next ❯

Citations

Sep 23, 2016·Journal of Biomaterials Science. Polymer Edition·Yomaira L UscáteguiManuel F Valero
Jan 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yomaira L UscáteguiManuel F Valero

❮ Previous
Next ❯

Methods Mentioned

BETA
chemical modification
chemical treatment
chips
fluorescence microscopy
Fluorescence

Software Mentioned

Image

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.