One stone, three birds: one AIEgen with three colors for fast differentiation of three pathogens.

Chemical Science
Chengcheng ZhouBen Zhong Tang

Abstract

Visually identifying pathogens favors rapid diagnosis at the point-of-care testing level. Here, we developed a microenvironment-sensitive aggregation-induced emission luminogen (AIEgen), namely IQ-Cm, for achieving fast discrimination of Gram-negative bacteria, Gram-positive bacteria and fungi by the naked-eye. With a twisted donor-acceptor and multi-rotor structure, IQ-Cm shows twisted intramolecular charge transfer (TICT) and AIE properties with sensitive fluorescence color response to the microenvironment of pathogens. Driven by the intrinsic structural differences of pathogens, IQ-Cm with a cationic isoquinolinium moiety and a membrane-active coumarin unit as the targeting and interacting groups selectively locates in different sites of three pathogens and gives three naked-eye discernible emission colors. Gram-negative bacteria are weak pink, Gram-positive bacteria are orange-red and fungi are bright yellow. Therefore, based on their distinctive fluorescence response, IQ-Cm can directly discriminate the three pathogens at the cell level under a fluorescence microscope. Furthermore, we demonstrated the feasibility of IQ-Cm as a visual probe for fast diagnosis of urinary tract infections, timely monitoring of hospital-acquir...Continue Reading

References

Jul 1, 1984·Antimicrobial Agents and Chemotherapy·R E Hancock, P G Wong
May 15, 1999·Journal of Clinical Microbiology·B S Reisner, G L Woods
Aug 21, 2001·Critical Care : the Official Journal of the Critical Care Forum·M H Kollef
Jan 10, 2002·Infectious Disease Clinics of North America·R B Thomson, H Bertram
Aug 19, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert GaynesUNKNOWN National Nosocomial Infections Surveillance System
Nov 28, 2006·Best Practice & Research. Clinical Rheumatology·Anja Strangfeld, Joachim Listing
May 18, 2007·Nature·Nathan D WolfeJared Diamond
Jun 29, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Heike Sträuber, Susann Müller
Aug 25, 2010·Journal of the American Chemical Society·Aidee DuarteGuillermo C Bazan
Sep 17, 2010·Journal of Biological Engineering·Mark A Haidekker, Emmanuel A Theodorakis
Feb 5, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kalpana GuptaUNKNOWN European Society for Microbiology and Infectious Diseases
Dec 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Ying WangDavid G Whitten
May 25, 2012·Journal of Microscopy·W Becker
Aug 8, 2012·Nature Reviews. Genetics·Xavier DidelotDerrick W Crook
Jun 8, 2013·Accounts of Chemical Research·Dan DingBen Zhong Tang
Jul 1, 1992·World Journal of Microbiology & Biotechnology·D Claus
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Peyton ShiehCarolyn R Bertozzi
Apr 17, 2014·Advanced Materials·Huanxiang YuanShu Wang
Jun 18, 2014·Bioorganic & Medicinal Chemistry·Sonali SandhuGulshan Bansal
Dec 17, 2014·Nature·Arturo Casadevall, Liise-anne Pirofski
Mar 21, 2015·Intensive Care Medicine·Matteo BassettiGaryphallia Poulakou
Oct 20, 2015·Nanoscale·Lulin YanWenjing Tian
Oct 23, 2015·Chemical Reviews·Ju MeiBen Zhong Tang
Oct 28, 2016·ACS Applied Materials & Interfaces·Haotian BaiShu Wang
Dec 13, 2016·Chemical Society Reviews·Juhong ChenVincent M Rotello
Oct 4, 2017·Chemical Science·Meijuan JiangBen Zhong Tang
Nov 16, 2017·ACS Applied Materials & Interfaces·Ju MeiHe Tian
Jan 7, 2018·American Journal of Obstetrics and Gynecology·Christine M Chu, Jerry L Lowder
Mar 2, 2018·Science Translational Medicine·Mireille KamarizaCarolyn R Bertozzi
Apr 27, 2019·Angewandte Chemie·Haw-Young KwonYoung-Tae Chang
Jan 1, 2010·Comprehensive Reviews in Food Science and Food Safety·Rajeev BhatA A Karim

❮ Previous
Next ❯

Citations

Sep 23, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ming-Yu WuShun Feng
Dec 16, 2020·Biomaterials·Haotian BaiBen Zhong Tang
Apr 12, 2021·Journal of Colloid and Interface Science·Pablo G ArgudoJuan J Giner-Casares
Aug 4, 2021·Advanced Healthcare Materials·Qinggele BorjihanYing-Wei Yang
Jan 12, 2021·Journal of the American Chemical Society·Adam C SedgwickJonathan L Sessler

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
Fluorescence
fluorescence imaging
fluorescence microscopy
transmission electron diffraction

Software Mentioned

IQ
Gaussian
Cm

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.