One-Week Exposure to a Free-Choice High-Fat High-Sugar Diet Does Not Interfere With the Lipopolysaccharide-Induced Acute Phase Response in the Hypothalamus of Male Rats

Frontiers in Endocrinology
Evita BelegriAnita Boelen

Abstract

Obesity has been associated with increased susceptibility to infection in humans and rodents. Obesity is also associated with low-grade hypothalamic inflammation that depends not only on body weight but also on diet. In the present study, we investigated if the bacterial endotoxin [lipopolysaccharide (LPS)]-induced acute phase response is aggravated in rats on a 1-week free-choice high-fat high-sugar (fcHFHS) diet and explained by diet-induced hypothalamic inflammation. Male Wistar rats were on an fcHFHS diet or chow for 1 week and afterwards intraperitoneally injected with LPS or saline. Hypothalamic inflammatory intermediates and plasma cytokines were measured after LPS. Both LPS and the fcHFHS diet altered hypothalamic Nfkbia mRNA and nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha (NFKBIA) protein levels, whereas Il1β, Il6, and Tnfα mRNA expression was solely induced upon LPS. We observed an interaction in hypothalamic Nfkbia and suppressor of cytokine signaling (SOCS) 3 mRNA upon LPS; both were higher in rats on a fcHFHS diet compared with chow animals. Despite this, plasma cytokine levels between fcHFHS diet-fed and chow-fed rats were similar after LPS administration. Consuming a fcHFHS ...Continue Reading

References

Dec 24, 1997·Journal of the American College of Surgeons·P S Choban, L Flancbaum
Feb 23, 2000·The Journal of Clinical Investigation·E LienD T Golenbock
Dec 20, 2002·Nature·Jonathan Cohen
Jun 14, 2003·Obesity Research·Zeynep CantürkIlhan Tarkun
Aug 18, 2004·International Journal of Clinical Practice·A S Jubber
May 4, 2005·The Journal of Allergy and Clinical Immunology·Giamila Fantuzzi
Apr 21, 2006·Immunological Reviews·Alexander Hoffmann, David Baltimore
Jun 23, 2006·The Lancet Infectious Diseases·Matthew E Falagas, Maria Kompoti
Aug 19, 2006·Nature Reviews. Immunology·Nicholas J GayAlexander N R Weber
May 26, 2007·Nature Reviews. Immunology·Akihiko YoshimuraMasato Kubo
Jan 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marciane MilanskiLício A Velloso
Apr 1, 2010·American Journal of Physiology. Endocrinology and Metabolism·Sun Ju ChoiBrent E Wisse
Jan 27, 2011·Methods in Enzymology·Christine M Oslowski, Fumihiko Urano
Mar 10, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·L A Velloso, M W Schwartz
Dec 29, 2011·The Journal of Clinical Investigation·Joshua P ThalerMichael W Schwartz
Feb 14, 2012·Disease Models & Mechanisms·Catherine B LawrenceElysse M Knight
Feb 14, 2012·Aging·Dongsheng Cai, Tiewen Liu
Dec 11, 2013·The Journal of Biological Chemistry·Jaemin Lee, Umut Ozcan
Apr 1, 2015·Nature Reviews. Endocrinology·Stefanie KälinChun-Xia Yi
Nov 7, 2015·Seminars in Cell & Developmental Biology·Kevin G BurfeindDaniel L Marks
Dec 1, 2015·Journal of Neuroimmunology·Lidiane OrlandiAlexandre Giusti-Paiva
May 31, 2017·Frontiers in Neuroscience·Evita BelegriSusanne E la Fleur

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

LinRegPCR
ImageJ
Primer Blast
SPSS

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.