One Year Genome Evolution of Lausannevirus in Allopatric versus Sympatric Conditions

Genome Biology and Evolution
Linda MuellerGilbert Greub

Abstract

Amoeba-resisting microorganisms raised a great interest during the last decade. Among them, some large DNA viruses present huge genomes up to 2.5 Mb long, exceeding the size of small bacterial genomes. The rate of genome evolution in terms of mutation, deletion, and gene acquisition in these genomes is yet unknown. Given the suspected high plasticity of viral genomes, the microevolution of the 346 kb genome of Lausannevirus, a member of Megavirales, was studied. Hence, Lausannevirus was co-cultured within the amoeba Acanthamoeba castellanii over one year. Despite a low number of mutations, the virus showed a genome reduction of 3.7% after 12 months. Lausannevirus genome evolution in sympatric conditions was investigated by its co-culture with Estrella lausannensis, an obligate intracellular bacterium, in the amoeba A. castellanii during one year. Cultures were split every 3 months. Genome sequencing revealed that in these conditions both, Lausannevirus and E. lausannensis, show stable genome, presenting no major rearrangement. In fact, after one year they acquired from 2 to 7 and from 4 to 10 mutations per culture for Lausannevirus and E. lausannensis, respectively. Interestingly, different mutations in the endonuclease encodin...Continue Reading

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Citations

May 29, 2018·Microbes and Environments·Tomoko MiharaHiroyuki Ogata
Jun 20, 2019·Nucleic Acids Research·Alexandra ZinovievChristopher U T Hellen

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Datasets Mentioned

BETA
PRJEB7018
PRJEB20258

Methods Mentioned

BETA
PCR
Assay
phosphotransferase

Software Mentioned

QUAST
Phyre2
VarScan
BLAST
BLASTN
Burrows Aligner ( BWA )
iPython
SPAdes
Insectomime
Sequence Alignment / Map ( SAM ) Tools

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