Online autofluorescence measurements during selective RPE laser treatment

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
C FrammeR Brinkmann

Abstract

Fundus autofluorescence (AF) is derived from the lipofuscin contained by the retinal pigment epithelial cells. Using a scanning laser ophthalmoscope, two-dimensional AF measurements of the ocular fundus can be achieved. Directly after conventional photocoagulation and also after selective RPE laser treatment (SRT) with ophthalmoscopically non-visible laser lesions, irradiated areas reveal reduced AF, indicating RPE damage. Since the green treatment laser beam could also be used for AF excitation, the aim of this study was to evaluate whether absolute measurements of AF can be performed, and also possible changes in AF detected, online during SRT. SRT was carried out by use of a frequency-doubled Nd:YLF laser (wavelength 527 nm, pulse duration 1.7 micros, repetition rate 500 and 100 Hz, number of pulses 100 and 30, single pulse energy 50-130 microJ) in vitro (porcine RPE; retinal spot size 160 microm) and during patient treatment (retinal spot size 176 microm). During irradiation, fluorescence light from the RPE was decoupled from the laser light inside the slit lamp and detected by a photomultiplier or photodiode at wavelengths above 550 nm. Additionally, temperature-dependent fluorescence intensity measurements of A2-E, the ma...Continue Reading

Citations

Sep 26, 2008·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·A E HöhS Dithmar
May 25, 2005·Photomedicine and Laser Surgery
Jun 16, 2005·Photomedicine and Laser Surgery
Sep 8, 2005·Photomedicine and Laser Surgery
Apr 15, 2008·Investigative Ophthalmology & Visual Science·Jessica I W MorganDavid R Williams
Sep 21, 2010·Survey of Ophthalmology·Sobha SivaprasadGiorgio Dorin
Mar 11, 2008·Retina·Steffen Schmitz-ValckenbergRichard F Spaide
Nov 15, 2011·European Journal of Ophthalmology·Daniele VerittiPaolo Lanzetta

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