Online CO2 and H2 O oxygen isotope fractionation allows estimation of mesophyll conductance in C4 plants, and reveals that mesophyll conductance decreases as leaves age in both C4 and C3 plants

The New Phytologist
Margaret M BarbourSusanne von Caemmerer

Abstract

Mesophyll conductance significantly, and variably, limits photosynthesis but we currently have no reliable method of measurement for C4 plants. An online oxygen isotope technique was developed to allow quantification of mesophyll conductance in C4 plants and to provide an alternative estimate in C3 plants. The technique is compared to an established carbon isotope method in three C3 species. Mesophyll conductance of C4 species was similar to that in the C3 species measured, and declined in both C4 and C3 species as leaves aged from fully expanded to senescing. In cotton leaves, simultaneous measurement of carbon and oxygen isotope discrimination allowed the partitioning of total conductance to the chloroplasts into cell wall and plasma membrane versus chloroplast membrane components, if CO2 was assumed to be isotopically equilibrated with cytosolic water, and the partitioning remained stable with leaf age. The oxygen isotope technique allowed estimation of mesophyll conductance in C4 plants and, when combined with well-established carbon isotope techniques, may provide additional information on mesophyll conductance in C3 plants.

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Citations

Oct 6, 2016·Journal of Experimental Botany·Hannah L OsbornSusanne von Caemmerer
Nov 20, 2016·Plant, Cell & Environment·Karen E LoucosMargaret M Barbour
Jan 23, 2017·Journal of Experimental Botany·Susanne von CaemmererRobert T Furbank
Oct 19, 2017·Journal of Experimental Botany·Balasaheb V SonawaneOula Ghannoum
Dec 23, 2017·The New Phytologist
May 16, 2019·The New Phytologist·Francisco Javier CanoOula Ghannoum
Feb 14, 2019·Photosynthesis Research·Haoran ZhouBrent R Helliker
Sep 24, 2019·The New Phytologist·Yong LiMargaret M Barbour
Oct 30, 2019·The Plant Journal : for Cell and Molecular Biology·Jürgen KnauerYing Sun
Dec 20, 2019·The Plant Journal : for Cell and Molecular Biology·Marjorie R Lundgren, Andrew J Fleming
Jan 22, 2020·The Plant Journal : for Cell and Molecular Biology·Asaph B CousinsBalasaheb V Sonawane
Feb 11, 2020·Photosynthesis Research·Xinyou YinPaul C Struik
Mar 5, 2020·Biochemical Society Transactions·Jorge GagoJaume Flexas
Feb 16, 2020·Journal of Experimental Botany·Xin-Guang ZhuSusanne von Caemmerer
Mar 1, 2020·The New Phytologist·Erika A Serrano-Romero, Asaph B Cousins
May 22, 2020·The Plant Journal : for Cell and Molecular Biology·Balasaheb V Sonawane, Asaph B Cousins
Oct 6, 2018·Nature Plants·Coralie E Salesse-SmithDavid B Stern
Mar 13, 2016·Journal of Experimental Botany·Xinyou YinPaul C Struik
May 16, 2017·Journal of Experimental Botany·John A RavenPatricia Sánchez-Baracaldo
Jul 9, 2016·Plant, Cell & Environment·Meisha Holloway-PhillipsGraham D Farquhar
Jan 4, 2017·Journal of Experimental Botany·John A Raven
Sep 16, 2017·Journal of Experimental Botany·Anthony William D LarkumJohn A Raven
Nov 3, 2020·The New Phytologist·John R Evans
Feb 26, 2021·Interface Focus·Victoria C ClarkeSusanne von Caemmerer
Mar 20, 2021·Journal of Experimental Botany·Raed ElferjaniMebarek Lamara

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