Ontogenesis of myosin light chain kinase mRNA and protein content in guinea pig tracheal smooth muscle

Pediatric Pulmonology
Pasquale ChitanoThomas M Murphy

Abstract

We previously reported in guinea pig tracheal smooth muscle that maximal shortening velocity decreases from 3 weeks of age to adulthood. It is not known whether myosin light chain kinase (MLCK), a key enzyme determining the velocity of smooth muscle contraction, undergoes maturational changes. In the present work, we investigated MLCK protein content and mRNA expression in 1-week-old, 3-week-old, and adult guinea pigs. We extracted either proteins or RNA from isolated tracheal smooth muscle. The content of MLCK was assessed by Western immunoblots. MLCK mRNA was evaluated by Northern analysis and by quantitative real time reverse transcriptase-polymerase chain reaction (RT-PCR). The content of MLCK increased 3-fold at 3 weeks of age and then decreased in adults, being 0.116 +/- 0.042, 0.330 +/- 0.125 (P < 0.05), and 0.153 +/- 0.054 microg/mg of total protein, respectively, in 1-week, 3-week, and adult animals. Quantitative RT-PCR revealed that MLCK mRNA increased with age to 135 +/- 35% and 177 +/- 23% (P < 0.01) in 3-week and adult animals, respectively, compared to 1-week animals. The transient increase of MLCK content in juvenile guinea pig tracheal smooth muscle may contribute to the increased shortening velocity at this age...Continue Reading

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Citations

Oct 7, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Elisha R InjetiWilliam J Pearce
Apr 23, 2011·Pulmonary Pharmacology & Therapeutics·Pasquale Chitano
Sep 16, 2010·British Journal of Pharmacology·Stefano FogliMaria Cristina Breschi
Jan 29, 2014·Fitoterapia·Yong-Jian XiongYuan Lin
Mar 19, 2014·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Fang-Fei LiuYuan Lin
Jan 10, 2017·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Changchun YuLi Wang
Dec 8, 2005·Canadian Journal of Physiology and Pharmacology·Pasquale ChitanoThomas M Murphy
Dec 2, 2004·Pediatric Pulmonology·Pasquale ChitanoThomas M Murphy
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Sep 8, 2007·Canadian Journal of Physiology and Pharmacology·Pasquale ChitanoThomas M Murphy
Sep 12, 2019·Frontiers in Physiology·Peter B NobleKimberley C W Wang
Jun 26, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bernard M FischerJudith A Voynow
Oct 29, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dane W SorensenWilliam J Pearce
Jul 29, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Dane W SorensenWilliam J Pearce

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