PMID: 3756504Sep 3, 1986Paper

Ontogenesis of nuclear T3 receptors in primary cultured astrocytes and neurons

Brain Research
M LuoJ H Dussault

Abstract

Nuclear T3 receptor (NTR) have been characterized in separated cultures of neurons and astrocytes. Scatchard analysis indicated the presence of a single class of high-affinity sites in both cell lines. The apparent equilibrium association constant ranged from 1.80 +/- 0.41 X 10(10) M-1 to 3.27 +/- 0.74 +/- 10(10) M-1 in neurons and from 1.01 +/- 0.09 to 1.80 +/- 0.73 X 10(10) M-1 in astrocytes depending on the time in culture. In neurons, the maximal binding capacity (MBC) increased from 0.049 +/- 0.008 ng T3/mg DNA to 0.328 +/- 0.052 ng T3/mg DNA between 3 and 12 days of culture. In astrocytes, the changes in MBC were less pronounced ranging from a minimum of 0.095 +/- 0.024 ng T3/mg DNA at the 7th day of culture to a maximum of 0.198 +/- 0.048 ng T3/mg DNA at the 21st day. The relative binding affinity of the receptor for thyroid hormone analogs was in the order TRIAC greater than L-T3 greater than D-T3 greater than L-T4 in both cell lines. These results show that nuclear T3 receptors similar to those found in vivo are present in primary cultures of both astrocytes and neurons.

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Citations

Mar 1, 1989·Brain Research. Developmental Brain Research·M LuoJ H Dussault
Jan 15, 1991·Brain Research. Developmental Brain Research·J M GavaretM Pierre
Oct 23, 1992·Brain Research. Developmental Brain Research·J PuymiratJ H Dussault
Sep 1, 1992·Progress in Neurobiology·J Puymirat
Mar 5, 1990·Molecular and Cellular Endocrinology·E GoncalvesJ Robbins
Feb 12, 2002·Neuroscience and Biobehavioral Reviews·Jeremy W SmithJames W Smythe
Apr 9, 2002·Journal of Endocrinological Investigation·J Bernal
Jan 1, 1997·Brain Research Bulletin·P PerettoL Bonfanti
Feb 11, 2015·Pediatric Neurology·Debika Nandi-Munshi, Craig E Taplin
Jun 4, 2017·The Journal of Endocrinology·Stefan GroenewegW Edward Visser
Oct 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·C AnderssonJ Brunso-Bechtold
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity·Allan J WilliamsGraham R Williams

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